Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to protect the cell against oxidative stress. Expression of the Saccharomyces cerevisiae GSH1 gene, coding for the first enzyme involved in glutathione biosynthesis, is regulated at the level of transcription by oxidants and heavy metals. We have characterised the sequences of the GSH1 promoter responsible for the amino acid-dependent H2O2 regulation of transcription. We show that there are at least two H2O2-responsive elements in the promoter, neither of which map to the putative Yap1 binding site. Our results suggest that the Yap1 protein plays an important, but indirect role in the H2O2-dependent regulation of GSH1 transcription. © 2002 Elsevier Science B.V. All rights reserved.
|Number of pages||7|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|Publication status||Published - 7 Jun 2002|
- H 2 O 2
- Oxidative stress
- Saccharomyces cerevisiae