Oxidant regulation of the Saccharomyces cerevisiae GSH1 gene

Ulla H. Dormer, John Westwater, D. W S Stephen, Derek J. Jamieson

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to protect the cell against oxidative stress. Expression of the Saccharomyces cerevisiae GSH1 gene, coding for the first enzyme involved in glutathione biosynthesis, is regulated at the level of transcription by oxidants and heavy metals. We have characterised the sequences of the GSH1 promoter responsible for the amino acid-dependent H2O2 regulation of transcription. We show that there are at least two H2O2-responsive elements in the promoter, neither of which map to the putative Yap1 binding site. Our results suggest that the Yap1 protein plays an important, but indirect role in the H2O2-dependent regulation of GSH1 transcription. © 2002 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)23-29
    Number of pages7
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume1576
    Issue number1-2
    DOIs
    Publication statusPublished - 7 Jun 2002

    Keywords

    • Glutathione
    • H 2 O 2
    • Oxidative stress
    • Saccharomyces cerevisiae
    • Yap1

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