Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH

Yingbin Zhong, Xiao H. Zhang, Jixiang Chen, Zhenghao Chi, Boguang Sun, Yun Li, Brian Austin

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    36 Citations (Scopus)

    Abstract

    Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

    Original languageEnglish
    Pages (from-to)6001-6005
    Number of pages5
    JournalInfection and Immunity
    Volume74
    Issue number10
    DOIs
    Publication statusPublished - Oct 2006

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  • Cite this

    Zhong, Y., Zhang, X. H., Chen, J., Chi, Z., Sun, B., Li, Y., & Austin, B. (2006). Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH. Infection and Immunity, 74(10), 6001-6005. https://doi.org/10.1128/IAI.00512-06