Overexpression, pruification, characterisation and pathogenicity of Vibrio harveyi hemolysin VHH

Yingbin Zhong; Xiao-Hua Zhang; Jixiang Chen; Zhenghao Chi; Boguang Sun; Yun Li; Brian Austin

doi:10.1128/iai.00512-06

Overexpression, pruification, characterisation and pathogenicity of Vibrio harveyi hemolysin VHH

Yingbin Zhong
, Xiao-Hua Zhang
, Jixiang Chen
, Zhenghao Chi
, Boguang Sun
, Yun Li
, Brian Austin

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

Original language	English
Pages (from-to)	6001-6005
Number of pages	5
Journal	Infection and Immunity
Volume	74
Issue number	10
DOIs	https://doi.org/10.1128/iai.00512-06
Publication status	Published - Oct 2006

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title = "Overexpression, pruification, characterisation and pathogenicity of Vibrio harveyi hemolysin VHH",

abstract = "Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.",

author = "Yingbin Zhong and Xiao-Hua Zhang and Jixiang Chen and Zhenghao Chi and Boguang Sun and Yun Li and Brian Austin",

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T1 - Overexpression, pruification, characterisation and pathogenicity of Vibrio harveyi hemolysin VHH

AU - Zhong, Yingbin

AU - Zhang, Xiao-Hua

AU - Chen, Jixiang

AU - Chi, Zhenghao

AU - Sun, Boguang

AU - Li, Yun

AU - Austin, Brian

PY - 2006/10

Y1 - 2006/10

N2 - Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

AB - Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

UR - https://www.scopus.com/pages/publications/33749242440

U2 - 10.1128/iai.00512-06

DO - 10.1128/iai.00512-06

M3 - Article

C2 - 16988279

SN - 0019-9567

VL - 74

SP - 6001

EP - 6005

JO - Infection and Immunity

JF - Infection and Immunity

IS - 10

ER -

Overexpression, pruification, characterisation and pathogenicity of Vibrio harveyi hemolysin VHH

Abstract

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