TY - JOUR
T1 - OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems
AU - Mendonca, Tania
AU - Lis-Slimak, Katarzyna
AU - Matheson, Andrew B.
AU - Smith, Matthew G.
AU - Anane-Adjei, Akosua B.
AU - Ashworth, Jennifer C.
AU - Cavanagh, Robert
AU - Paterson, Lynn
AU - Dalgarno, Paul A.
AU - Alexander, Cameron
AU - Tassieri, Manlio
AU - Merry, Catherine L. R.
AU - Wright, Amanda J.
N1 - Funding Information:
The authors acknowledge support via linked EPSRC grants EP/R035067/1, EP/R035563/1, and EP/R035156/1, pilot grant funding from Nottingham Breast Cancer Research Centre, Anne McLaren fellowship funding from the University of Nottingham (JCA) and NC3Rs grants NC/T001259/1 and NC/T001267/1. The MCF-7 eGFP, MCF7-tdTomato and MDA-MB-231 tdTomato cell lines were provided by and with thanks to Prof. Anna Grabowska, University of Nottingham.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/4/28
Y1 - 2023/4/28
N2 - Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named ‘OptoRheo’ that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing the mechanical properties of the surrounding extracellular and pericellular matrix at a sub-cellular length scale. OptoRheo can be used in two operational modalities (with and without an optical trap) to extend the dynamic range of microrheology measurements. We corroborated this by characterising the ECM surrounding live breast cancer cells in two distinct culture systems, cell clusters in 3D hydrogels and spheroids in suspension culture. This cutting-edge instrument will transform the exploration of drug transport through complex cell culture matrices and optimise the design of the next-generation of disease models.
AB - Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named ‘OptoRheo’ that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing the mechanical properties of the surrounding extracellular and pericellular matrix at a sub-cellular length scale. OptoRheo can be used in two operational modalities (with and without an optical trap) to extend the dynamic range of microrheology measurements. We corroborated this by characterising the ECM surrounding live breast cancer cells in two distinct culture systems, cell clusters in 3D hydrogels and spheroids in suspension culture. This cutting-edge instrument will transform the exploration of drug transport through complex cell culture matrices and optimise the design of the next-generation of disease models.
KW - Cell Communication
KW - Extracellular Matrix
KW - Hydrogels
KW - Microscopy, Fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85157981722&partnerID=8YFLogxK
U2 - 10.1038/s42003-023-04780-8
DO - 10.1038/s42003-023-04780-8
M3 - Article
C2 - 37117487
SN - 2399-3642
VL - 6
JO - Communications Biology
JF - Communications Biology
M1 - 463
ER -
