OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing

T. Mendonca; K. Lis-Slimak; A. B. Matheson; M. G. Smith; A. B. Anane-Adjei; J. C. Ashworth; R. Cavanagh; L. Paterson; P. A. Dalgarno; C. Alexander; M. Tassieri; C. L. R. Merry; A. J. Wright

doi:10.1051/epjconf/202533504013

OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing

T. Mendonca
, K. Lis-Slimak
, A. B. Matheson
, M. G. Smith
, A. B. Anane-Adjei
, J. C. Ashworth
, R. Cavanagh
, L. Paterson
, P. A. Dalgarno
, C. Alexander
, M. Tassieri
, C. L. R. Merry
, A. J. Wright

Research output: Contribution to journal › Conference article › peer-review

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Abstract

OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.

Original language	English
Article number	04013
Journal	EPJ Web of Conferences
Volume	335
DOIs	https://doi.org/10.1051/epjconf/202533504013
Publication status	Published - 22 Sept 2025
Event	2025 European Optical Society Annual Meeting - Delft, Netherlands Duration: 24 Aug 2025 → 28 Aug 2025

ASJC Scopus subject areas

General Physics and Astronomy

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Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Ashworth, J. C., Cavanagh, R., Paterson, L., Dalgarno, P. A., Alexander, C., Tassieri, M., Merry, C. L. R., & Wright, A. J. (2025). OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing. EPJ Web of Conferences, 335, Article 04013. https://doi.org/10.1051/epjconf/202533504013

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title = "OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing",

abstract = "OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.",

author = "T. Mendonca and K. Lis-Slimak and Matheson, \{A. B.\} and Smith, \{M. G.\} and Anane-Adjei, \{A. B.\} and Ashworth, \{J. C.\} and R. Cavanagh and L. Paterson and Dalgarno, \{P. A.\} and C. Alexander and M. Tassieri and Merry, \{C. L. R.\} and Wright, \{A. J.\}",

note = "Publisher Copyright: {\textcopyright} The Authors, published by EDP Sciences, 2025.; 2025 European Optical Society Annual Meeting, EOSAM 2025 ; Conference date: 24-08-2025 Through 28-08-2025",

year = "2025",

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doi = "10.1051/epjconf/202533504013",

language = "English",

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TY - JOUR

T1 - OptoRheo

T2 - 2025 European Optical Society Annual Meeting

AU - Mendonca, T.

AU - Lis-Slimak, K.

AU - Matheson, A. B.

AU - Smith, M. G.

AU - Anane-Adjei, A. B.

AU - Ashworth, J. C.

AU - Cavanagh, R.

AU - Paterson, L.

AU - Dalgarno, P. A.

AU - Alexander, C.

AU - Tassieri, M.

AU - Merry, C. L. R.

AU - Wright, A. J.

PY - 2025/9/22

Y1 - 2025/9/22

N2 - OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.

AB - OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.

UR - https://www.scopus.com/pages/publications/105019049196

U2 - 10.1051/epjconf/202533504013

DO - 10.1051/epjconf/202533504013

M3 - Conference article

AN - SCOPUS:105019049196

SN - 2101-6275

VL - 335

JO - EPJ Web of Conferences

JF - EPJ Web of Conferences

M1 - 04013

Y2 - 24 August 2025 through 28 August 2025

ER -

OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing

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