Abstract
There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell.
| Original language | English |
|---|---|
| Journal | Journal of Biophotonics |
| Early online date | 9 May 2017 |
| DOIs | |
| Publication status | E-pub ahead of print - 9 May 2017 |
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Keywords
- Cell Mechanics
- Lamin A
- Nuclear Envelope
- Optical Stretcher
- Single-Cell Analysis
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
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Optomechanical measurement of the role of Lamins in whole cell deformability. / Kolb, Thorsten; Kraxner, Julia; Skodzek, Kai; Haug, Michael; Crawford, Dean; Maaß, Kendra K.; Aifantis, Katerina E.; Whyte, Graeme.
In: Journal of Biophotonics, 09.05.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Optomechanical measurement of the role of Lamins in whole cell deformability
AU - Kolb, Thorsten
AU - Kraxner, Julia
AU - Skodzek, Kai
AU - Haug, Michael
AU - Crawford, Dean
AU - Maaß, Kendra K.
AU - Aifantis, Katerina E.
AU - Whyte, Graeme
PY - 2017/5/9
Y1 - 2017/5/9
N2 - There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell.
AB - There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell.
KW - Cell Mechanics
KW - Lamin A
KW - Nuclear Envelope
KW - Optical Stretcher
KW - Single-Cell Analysis
UR - http://www.scopus.com/inward/record.url?scp=85019019208&partnerID=8YFLogxK
U2 - 10.1002/jbio.201600198
DO - 10.1002/jbio.201600198
M3 - Article
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
ER -