Optically enhanced acoustophoresis

Craig McDougall, Paul O'Mahoney, Alan McGuinn, Nicholas A. Willoughby, Yongqiang Qiu, Christine E. M. Demore, Michael P. MacDonald

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: Dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed «optically enhanced acoustophoresis» which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XIV
EditorsKishan Dholakia, Gabriel C. Spalding
PublisherSPIE
ISBN (Electronic)9781510611528
ISBN (Print)9781510611511
DOIs
Publication statusPublished - 25 Aug 2017

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10347
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Keywords

  • acoustophoresis
  • bioprocessing
  • Cell sorting
  • cell therapy
  • optical guiding
  • optical trapping

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    McDougall, C., O'Mahoney, P., McGuinn, A., Willoughby, N. A., Qiu, Y., Demore, C. E. M., & MacDonald, M. P. (2017). Optically enhanced acoustophoresis. In K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XIV [103471B] (Proceedings of SPIE; Vol. 10347). SPIE. https://doi.org/10.1117/12.2276323