We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The system allows the 3D tracking of an optically trapped bead, with ~ 20 nm accuracy along the optical axis. This is achieved without the need for a high precision z-stage, separate calibration sample, nor a priori knowledge of either the bead size or the optical properties of the suspending medium. Instead, we have developed a simple yet effective in situ spatial calibration method using image sharpness and exploiting the fact we image at multiple planes simultaneously. These features make OpTIMuM an ideal system for microrheology measurements, and we corroborate the effectiveness of this novel microrheology tool by measuring the viscosity of water in three dimensions, simultaneously.
ASJC Scopus subject areas
FingerprintDive into the research topics of 'Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM): a new tool for 3D microrheology'. Together they form a unique fingerprint.
Press / Media
Optoforce - bespoke new instrument for optical imaging, micro-mechanical-sensing and the chemical perturbation of 3D cell cultures
1 Media contribution