Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers

Tanya K. Lake; Antonia E. Carruthers; Lynn Paterson; Margaret Taylor; Frank Gunn-Moore; John W. Allen; Wilson Sibbett; Kishan Dholakia

doi:10.1364/OPEX.12.000670

Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers

Tanya K. Lake^*, Antonia E. Carruthers, Lynn Paterson, Margaret Taylor, Frank Gunn-Moore, John W. Allen, Wilson Sibbett, Kishan Dholakia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics.

Original language	English
Pages (from-to)	670-678
Number of pages	9
Journal	Optics Express
Volume	12
Issue number	4
DOIs	https://doi.org/10.1364/OPEX.12.000670
Publication status	Published - 23 Feb 2004

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OPEX.12.000670

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abstract = "Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics.",

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AU - Lake, Tanya K.

AU - Carruthers, Antonia E.

AU - Paterson, Lynn

AU - Taylor, Margaret

AU - Gunn-Moore, Frank

AU - Allen, John W.

AU - Sibbett, Wilson

AU - Dholakia, Kishan

PY - 2004/2/23

Y1 - 2004/2/23

N2 - Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics.

AB - Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4'-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics.

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VL - 12

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Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers

Abstract

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