Operating spiral inertial microfluidics at concentrations up to 3.4x109 cells/ml for high-concentration dewatering of chlorella vulgaris

Catherine Hill; Nik Willoughby; Helen Bridle

Operating spiral inertial microfluidics at concentrations up to 3.4x10⁹ cells/ml for high-concentration dewatering of chlorella vulgaris

Catherine Hill^*, Nik Willoughby, Helen Bridle

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Inertial focusing has been successfully applied to microalgal harvesting, predominantly utilizing low concentration cultures, with few examples greater than 1x10⁷ cells/ml, and with limited success when applied to small microalgal species (<10μm). High concentration processing using inertial focusing has introduced challenges in achieving successful, high recovery, separation due to particle interactions interfering with the focusing performance. Here we utilized a spiral channel with Chlorella vulgaris (CCAP 211/21A) and report successful operation across algae concentrations of 2.9x10⁷ - 3.4x10⁹ cells/ml in our device. Future work aims to further increase performance through device parallelization and scale-up.

Original language	English
Title of host publication	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	657-658
Number of pages	2
ISBN (Electronic)	9781733419017
Publication status	Published - 2020
Event	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2020 - Virtual, Online Duration: 4 Oct 2020 → 9 Oct 2020

Conference

Conference	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2020
Abbreviated title	MicroTAS 2020
City	Virtual, Online
Period	4/10/20 → 9/10/20

Keywords

Algal Dewatering/Harvesting
Inertial Focusing
Microalgae
Microfluidics

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering
General Chemistry
Control and Systems Engineering

Cite this

@inproceedings{b445ef7af5554535988fbb304ebc26c2,

title = "Operating spiral inertial microfluidics at concentrations up to 3.4x109 cells/ml for high-concentration dewatering of chlorella vulgaris",

abstract = "Inertial focusing has been successfully applied to microalgal harvesting, predominantly utilizing low concentration cultures, with few examples greater than 1x107 cells/ml, and with limited success when applied to small microalgal species (<10μm). High concentration processing using inertial focusing has introduced challenges in achieving successful, high recovery, separation due to particle interactions interfering with the focusing performance. Here we utilized a spiral channel with Chlorella vulgaris (CCAP 211/21A) and report successful operation across algae concentrations of 2.9x107 - 3.4x109 cells/ml in our device. Future work aims to further increase performance through device parallelization and scale-up.",

keywords = "Algal Dewatering/Harvesting, Inertial Focusing, Microalgae, Microfluidics",

author = "Catherine Hill and Nik Willoughby and Helen Bridle",

year = "2020",

language = "English",

pages = "657--658",

booktitle = "24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2020, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",

}

Hill, C, Willoughby, N & Bridle, H 2020, Operating spiral inertial microfluidics at concentrations up to 3.4x10⁹ cells/ml for high-concentration dewatering of chlorella vulgaris. in 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 657-658, 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2020, Virtual, Online, 4/10/20.

Operating spiral inertial microfluidics at concentrations up to 3.4x10⁹ cells/ml for high-concentration dewatering of chlorella vulgaris. / Hill, Catherine; Willoughby, Nik ; Bridle, Helen.
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2020. p. 657-658.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Operating spiral inertial microfluidics at concentrations up to 3.4x109 cells/ml for high-concentration dewatering of chlorella vulgaris

AU - Hill, Catherine

AU - Willoughby, Nik

AU - Bridle, Helen

PY - 2020

Y1 - 2020

N2 - Inertial focusing has been successfully applied to microalgal harvesting, predominantly utilizing low concentration cultures, with few examples greater than 1x107 cells/ml, and with limited success when applied to small microalgal species (<10μm). High concentration processing using inertial focusing has introduced challenges in achieving successful, high recovery, separation due to particle interactions interfering with the focusing performance. Here we utilized a spiral channel with Chlorella vulgaris (CCAP 211/21A) and report successful operation across algae concentrations of 2.9x107 - 3.4x109 cells/ml in our device. Future work aims to further increase performance through device parallelization and scale-up.

AB - Inertial focusing has been successfully applied to microalgal harvesting, predominantly utilizing low concentration cultures, with few examples greater than 1x107 cells/ml, and with limited success when applied to small microalgal species (<10μm). High concentration processing using inertial focusing has introduced challenges in achieving successful, high recovery, separation due to particle interactions interfering with the focusing performance. Here we utilized a spiral channel with Chlorella vulgaris (CCAP 211/21A) and report successful operation across algae concentrations of 2.9x107 - 3.4x109 cells/ml in our device. Future work aims to further increase performance through device parallelization and scale-up.

KW - Algal Dewatering/Harvesting

KW - Inertial Focusing

KW - Microalgae

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85098264972&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85098264972

SP - 657

EP - 658

BT - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2020

Y2 - 4 October 2020 through 9 October 2020

ER -