On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy

Julia Gala de Pablo; David R. Chisholm; Sally A. Peyman; John M. Girkin; Carrie A. Ambler; Andrew Whiting; Stephen D. Evans

On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy

Julia Gala de Pablo^*, David R. Chisholm, Sally A. Peyman, John M. Girkin, Carrie A. Ambler, Andrew Whiting, Stephen D. Evans

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Raman of live single-cells on-chip can help elucidate metabolic and viability changes induced by processes such as drug treatment. However, the strong background of the most common materials for microfluidic fabrication, such as polydimethylsiloxane (PDMS), can sometimes overcome the single cell signal. Here we report the use of confocal Raman spectroscopy to obtain live single-cell spectra on-chip, using least square fitting of background to minimize the PDMS contribution. As a proof of concept, we trapped SW480 colorectal cancer cells in Takeuchi style PDMS traps and measured the uptake of novel photosensitizer DC473 on the same cell, before and after incubation.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018
Publisher	Chemical and Biological Microsystems Society
Pages	1318-1321
Number of pages	4
ISBN (Electronic)	9781510897571
Publication status	Published - 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018 - Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	11/11/18 → 15/11/18

Keywords

Drug uptake
PDMS
Photosensitizer
Raman
Single-cell

ASJC Scopus subject areas

General Chemistry
Bioengineering
Chemical Engineering (miscellaneous)
Control and Systems Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@inproceedings{3af488a14b8a409ea534bae985032856,

title = "On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy",

abstract = "Raman of live single-cells on-chip can help elucidate metabolic and viability changes induced by processes such as drug treatment. However, the strong background of the most common materials for microfluidic fabrication, such as polydimethylsiloxane (PDMS), can sometimes overcome the single cell signal. Here we report the use of confocal Raman spectroscopy to obtain live single-cell spectra on-chip, using least square fitting of background to minimize the PDMS contribution. As a proof of concept, we trapped SW480 colorectal cancer cells in Takeuchi style PDMS traps and measured the uptake of novel photosensitizer DC473 on the same cell, before and after incubation.",

keywords = "Drug uptake, PDMS, Photosensitizer, Raman, Single-cell",

author = "{Gala de Pablo}, Julia and Chisholm, {David R.} and Peyman, {Sally A.} and Girkin, {John M.} and Ambler, {Carrie A.} and Andrew Whiting and Evans, {Stephen D.}",

note = "Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

language = "English",

pages = "1318--1321",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018",

publisher = "Chemical and Biological Microsystems Society",

}

Gala de Pablo, J, Chisholm, DR, Peyman, SA, Girkin, JM, Ambler, CA, Whiting, A & Evans, SD 2018, On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018. Chemical and Biological Microsystems Society, pp. 1318-1321, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018, Kaohsiung, Taiwan, Province of China, 11/11/18.

On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy. / Gala de Pablo, Julia; Chisholm, David R.; Peyman, Sally A. et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018. Chemical and Biological Microsystems Society, 2018. p. 1318-1321.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy

AU - Gala de Pablo, Julia

AU - Chisholm, David R.

AU - Peyman, Sally A.

AU - Girkin, John M.

AU - Ambler, Carrie A.

AU - Whiting, Andrew

AU - Evans, Stephen D.

PY - 2018

Y1 - 2018

N2 - Raman of live single-cells on-chip can help elucidate metabolic and viability changes induced by processes such as drug treatment. However, the strong background of the most common materials for microfluidic fabrication, such as polydimethylsiloxane (PDMS), can sometimes overcome the single cell signal. Here we report the use of confocal Raman spectroscopy to obtain live single-cell spectra on-chip, using least square fitting of background to minimize the PDMS contribution. As a proof of concept, we trapped SW480 colorectal cancer cells in Takeuchi style PDMS traps and measured the uptake of novel photosensitizer DC473 on the same cell, before and after incubation.

AB - Raman of live single-cells on-chip can help elucidate metabolic and viability changes induced by processes such as drug treatment. However, the strong background of the most common materials for microfluidic fabrication, such as polydimethylsiloxane (PDMS), can sometimes overcome the single cell signal. Here we report the use of confocal Raman spectroscopy to obtain live single-cell spectra on-chip, using least square fitting of background to minimize the PDMS contribution. As a proof of concept, we trapped SW480 colorectal cancer cells in Takeuchi style PDMS traps and measured the uptake of novel photosensitizer DC473 on the same cell, before and after incubation.

KW - Drug uptake

KW - PDMS

KW - Photosensitizer

KW - Raman

KW - Single-cell

UR - http://www.scopus.com/inward/record.url?scp=85079744151&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85079744151

SP - 1318

EP - 1321

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

On-chip single cell drug uptake tracking using microfluidic traps and Raman microspectroscopy

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Other files and links

Fingerprint

Cite this