Of microscopes and microbes: characterising a novel nutrient uptake system in Escherichia coli biofilms

Liam M. Rooney; Paul A. Hoskisson; Gail McConnell

Of microscopes and microbes: characterising a novel nutrient uptake system in Escherichia coli biofilms

Liam M. Rooney, Paul A. Hoskisson, Gail McConnell

Research output: Contribution to conference › Poster

Abstract

Biofilms pose a public health risk due to their ability to protect bacteria from mechanical, environmental and chemical factors. Thereby they can confer resistance to their constituent bacteria and serve as a vehicle for spread of antimicrobial resistance [1]. Previous studies have shown complex fractal patterning and chirality in multi-strain colony biofilms; however, the architecture and substucture of single-strain communities is somewhat understudied. We aim to use the Mesolens to image the previously unexplored internal architecture of an intact Escherichia coli colony biofilm to better understand spatiotemporal organisation of a live acterial community.

Original language	English
Publication status	Published - 7 Jul 2019
Event	8th Congress of European Microbiologists 2019 - SEC Centre, Glasgow, United Kingdom Duration: 7 Jul 2019 → 11 Jul 2019 https://fems2019.org/

Conference

Conference	8th Congress of European Microbiologists 2019
Abbreviated title	FEMS 2019
Country/Territory	United Kingdom
City	Glasgow
Period	7/07/19 → 11/07/19
Internet address	https://fems2019.org/

Keywords

biofilm
microscopy
mesolens
mesoscopy
live cell imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Of microscopes and microbes: characterising a novel nutrient uptake system in Escherichia coli biofilms",

abstract = "Biofilms pose a public health risk due to their ability to protect bacteria from mechanical, environmental and chemical factors. Thereby they can confer resistance to their constituent bacteria and serve as a vehicle for spread of antimicrobial resistance [1]. Previous studies have shown complex fractal patterning and chirality in multi-strain colony biofilms; however, the architecture and substucture of single-strain communities is somewhat understudied. We aim to use the Mesolens to image the previously unexplored internal architecture of an intact Escherichia coli colony biofilm to better understand spatiotemporal organisation of a live acterial community.",

keywords = "biofilm, microscopy, mesolens, mesoscopy, live cell imaging",

author = "Rooney, {Liam M.} and Hoskisson, {Paul A.} and Gail McConnell",

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language = "English",

note = "8th Congress of European Microbiologists 2019, FEMS 2019 ; Conference date: 07-07-2019 Through 11-07-2019",

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T1 - Of microscopes and microbes

T2 - 8th Congress of European Microbiologists 2019

AU - Rooney, Liam M.

AU - Hoskisson, Paul A.

AU - McConnell, Gail

PY - 2019/7/7

Y1 - 2019/7/7

N2 - Biofilms pose a public health risk due to their ability to protect bacteria from mechanical, environmental and chemical factors. Thereby they can confer resistance to their constituent bacteria and serve as a vehicle for spread of antimicrobial resistance [1]. Previous studies have shown complex fractal patterning and chirality in multi-strain colony biofilms; however, the architecture and substucture of single-strain communities is somewhat understudied. We aim to use the Mesolens to image the previously unexplored internal architecture of an intact Escherichia coli colony biofilm to better understand spatiotemporal organisation of a live acterial community.

AB - Biofilms pose a public health risk due to their ability to protect bacteria from mechanical, environmental and chemical factors. Thereby they can confer resistance to their constituent bacteria and serve as a vehicle for spread of antimicrobial resistance [1]. Previous studies have shown complex fractal patterning and chirality in multi-strain colony biofilms; however, the architecture and substucture of single-strain communities is somewhat understudied. We aim to use the Mesolens to image the previously unexplored internal architecture of an intact Escherichia coli colony biofilm to better understand spatiotemporal organisation of a live acterial community.

KW - biofilm

KW - microscopy

KW - mesolens

KW - mesoscopy

KW - live cell imaging

M3 - Poster

Y2 - 7 July 2019 through 11 July 2019

ER -

Of microscopes and microbes: characterising a novel nutrient uptake system in Escherichia coli biofilms

Abstract

Conference

Keywords

UN SDGs

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Cite this