Occurrence of Pectinatus and Megasphaera in the major UK breweries

A D Paradh, Anne Elizabeth Hill, Wilfrid James Mitchell

Research output: Contribution to journalArticle

Abstract

The occurrence of beer spoilage bacteria belonging to the genera Pectinatus and Megasphaera in ten major UK breweries was investigated. The sampling points were selected from fermentation areas, beer conditioning areas and beer bottling and canning sites. Multiplex PCR methodology was used for detection of three Pectinatus and three Megasphaera species using species specific primers. The presence of six Lactobacillus species was also examined. Overall, 117 samples were analysed from ten breweries; six samples were positive for the presence of Pectinatus species and three samples were positive for the presence of Megasphaera species, while 34 samples were positive for the presence of Lactobacillus species. Lactobacillus species appeared to be the major potential spoilage microorganisms. Although none of the actual beer samples were found to be positive for Pectinatus and Megasphaera species, their occurrence in aerobic brewery environments indicates sanitation problems and revealed the presence of highly established biofilms in some breweries.
Original languageEnglish
Pages (from-to)498-506
Number of pages8
JournalJournal of the Institute of Brewing
Volume117
Issue number4
DOIs
Publication statusPublished - 2011

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Megasphaera
Pectinatus
brewing industry
beers
Lactobacillus
sampling
bottling
canning
sanitation
biofilm
fermentation

Keywords

  • beer spoilage bacteria, Megasphaera, multiplex PCR, Pectinatus

Cite this

Paradh, A D ; Hill, Anne Elizabeth ; Mitchell, Wilfrid James. / Occurrence of Pectinatus and Megasphaera in the major UK breweries. In: Journal of the Institute of Brewing. 2011 ; Vol. 117, No. 4. pp. 498-506.
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Occurrence of Pectinatus and Megasphaera in the major UK breweries. / Paradh, A D; Hill, Anne Elizabeth; Mitchell, Wilfrid James.

In: Journal of the Institute of Brewing, Vol. 117, No. 4, 2011, p. 498-506.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Occurrence of Pectinatus and Megasphaera in the major UK breweries

AU - Paradh, A D

AU - Hill, Anne Elizabeth

AU - Mitchell, Wilfrid James

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AB - The occurrence of beer spoilage bacteria belonging to the genera Pectinatus and Megasphaera in ten major UK breweries was investigated. The sampling points were selected from fermentation areas, beer conditioning areas and beer bottling and canning sites. Multiplex PCR methodology was used for detection of three Pectinatus and three Megasphaera species using species specific primers. The presence of six Lactobacillus species was also examined. Overall, 117 samples were analysed from ten breweries; six samples were positive for the presence of Pectinatus species and three samples were positive for the presence of Megasphaera species, while 34 samples were positive for the presence of Lactobacillus species. Lactobacillus species appeared to be the major potential spoilage microorganisms. Although none of the actual beer samples were found to be positive for Pectinatus and Megasphaera species, their occurrence in aerobic brewery environments indicates sanitation problems and revealed the presence of highly established biofilms in some breweries.

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