Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography

Matthew J. Dalby; Mathis O. Riehle; Stephen J Yarwood; Chris D. W. Wilkinson; Adam S. G. Curtis

doi:10.1016/S0014-4827(02)00053-8

Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography

Matthew J. Dalby, Mathis O. Riehle, Stephen J Yarwood, Chris D. W. Wilkinson, Adam S. G. Curtis

Research output: Contribution to journal › Article › peer-review

306 Citations (Scopus)

Abstract

Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new tissue. Material surface morphology has strong effects on cell cytoskeleton and morphology, and it is thought that cells may react to the topography of collagen and surrounding cells during tissue embryology. A poorly understood area is, however, gene-level responses to topography. Thus, this paper used microarray to probe for consistent gene changes in response to lithographically produced topography (12.5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling.

Original language	English
Pages (from-to)	274-282
Number of pages	9
Journal	Experimental Cell Research
Volume	284
Issue number	2
DOIs	https://doi.org/10.1016/S0014-4827(02)00053-8
Publication status	Published - 1 Apr 2003

Keywords

Cell Adhesion
Cell Communication
Cell Culture Techniques
Cell Line, Transformed
Cell Nucleus
Cell Polarity
Cell Size
Cytoskeleton
Extracellular Matrix
Fibroblasts
Gene Expression Regulation
Humans
Oligonucleotide Array Sequence Analysis
Protein Biosynthesis
Signal Transduction
Tissue Engineering
Transcription, Genetic

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10.1016/S0014-4827(02)00053-8

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title = "Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography",

abstract = "Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new tissue. Material surface morphology has strong effects on cell cytoskeleton and morphology, and it is thought that cells may react to the topography of collagen and surrounding cells during tissue embryology. A poorly understood area is, however, gene-level responses to topography. Thus, this paper used microarray to probe for consistent gene changes in response to lithographically produced topography (12.5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling.",

keywords = "Cell Adhesion, Cell Communication, Cell Culture Techniques, Cell Line, Transformed, Cell Nucleus, Cell Polarity, Cell Size, Cytoskeleton, Extracellular Matrix, Fibroblasts, Gene Expression Regulation, Humans, Oligonucleotide Array Sequence Analysis, Protein Biosynthesis, Signal Transduction, Tissue Engineering, Transcription, Genetic",

author = "Dalby, {Matthew J.} and Riehle, {Mathis O.} and Yarwood, {Stephen J} and Wilkinson, {Chris D. W.} and Curtis, {Adam S. G.}",

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T2 - response to a micro-grooved topography

AU - Dalby, Matthew J.

AU - Riehle, Mathis O.

AU - Yarwood, Stephen J

AU - Wilkinson, Chris D. W.

AU - Curtis, Adam S. G.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new tissue. Material surface morphology has strong effects on cell cytoskeleton and morphology, and it is thought that cells may react to the topography of collagen and surrounding cells during tissue embryology. A poorly understood area is, however, gene-level responses to topography. Thus, this paper used microarray to probe for consistent gene changes in response to lithographically produced topography (12.5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling.

AB - Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new tissue. Material surface morphology has strong effects on cell cytoskeleton and morphology, and it is thought that cells may react to the topography of collagen and surrounding cells during tissue embryology. A poorly understood area is, however, gene-level responses to topography. Thus, this paper used microarray to probe for consistent gene changes in response to lithographically produced topography (12.5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling.

KW - Cell Adhesion

KW - Cell Communication

KW - Cell Culture Techniques

KW - Cell Line, Transformed

KW - Cell Nucleus

KW - Cell Polarity

KW - Cell Size

KW - Cytoskeleton

KW - Extracellular Matrix

KW - Fibroblasts

KW - Gene Expression Regulation

KW - Humans

KW - Oligonucleotide Array Sequence Analysis

KW - Protein Biosynthesis

KW - Signal Transduction

KW - Tissue Engineering

KW - Transcription, Genetic

U2 - 10.1016/S0014-4827(02)00053-8

DO - 10.1016/S0014-4827(02)00053-8

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SP - 274

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography

Abstract

Keywords

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