Nontargeted LC-MSn profiling of compounds in ileal fluids that decrease after raspberry intake identifies consistent alterations in bile acid composition

Gordon J. McDougall, J. William Allwood, Gema Pereira-Caro, Emma M Brown, Nigel Ternan, Susan Verrall, Derek Stewart, Roger Lawther, Gloria O'Connor, Ian Rowland, Alan Crozier, Chris I. R. Gill

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ileostomy studies provide a unique insight into the digestion of foods, allowing identification of physiologically relevant dietary phytochemicals and their metabolites that are important to gut health. We previously reported an increase of components, including novel triterpenoids, in ileal fluids of 11 ileostomates following consumption of raspberries using nontargeted LC-MSn techniques in combination with data deconvolution software. The current study focused on components that consistently decreased postsupplementation. After data deconvolution, 32 components were identified that met exclusion parameters of m/z signals and which decreased significantly in ileal fluids from eight of 11 participants post-raspberry supplementation. Two-thirds of these components were identified putatively from their MS properties. Consistent decreases were observed in components that possibly reflected "washing out" of presupplementation intake of common foods/drinks including (poly)phenol metabolites. Metabolites associated with fat metabolism such as hydroxylated fatty acids and cholate-type bile acids were specifically reduced. However, more directed re-examination of the data revealed that although some cholates were consistently reduced, the more polar glyco- and tauro-linked bile acid derivatives increased consistently, by as much as 100-fold over presupplementation levels. The possible reasons for these substantial alterations in bile acid composition in ileal fluids in response to raspberry intake are discussed.

Original languageEnglish
Pages (from-to)2606-2615
Number of pages10
JournalJournal of Natural Products
Volume79
Issue number10
Early online date19 Sep 2016
DOIs
Publication statusPublished - 28 Oct 2016

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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