Non-invasive Detection Method for Bonamia ostreae in Ostrea edulis using the Franklin qPCR machine V.1

This is a comprehensive protocol for using the Franklin qPCR (Biomeme Inc.) machine to detect the presence of Bonamia ostreae parasite in European flat oyster (Ostrea edulis). Previous methods relied on sacrificially dissecting animals to perform histology. While sensitive, these methods were destructive and untested animals carrying Bonamia ostreae are not detected. Here, we present a non-invasive alternative method to detect Bonamia ostreae from Ostrea edulis pseudofaeces/faeces using a portable qPCR machine.