Abstract
Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 379-381 |
| Number of pages | 3 |
| Journal | Inorganica Chimica Acta |
| Volume | 356 |
| DOIs | |
| Publication status | Published - 3 Dec 2003 |
Keywords
- ENERGY TRANSDUCTION
- paramagnetic shifts
- DESULFOVIBRIO-VULGARIS
- multihaem
- RESPIRATION
- Shewanella
- electron tranfer protein
- PUTREFACIENS MR-1
- ELECTRON-TRANSFER
- COOPERATIVITY
- flavocytochrome
- NCIMB400
- NMR
- FUMARATE REDUCTASE
- CYTOCHROME C(3)
- HEME CORE
Cite this
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NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina. / Pessanha, Miguel; Turner, David L; Rothery, Emma L; Pankhurst, Katherine L; Reid, Graeme A; Chapman, Stephen K; Xavier, Antonio V; Salgueiro, Carlos A.
In: Inorganica Chimica Acta, Vol. 356, 03.12.2003, p. 379-381.Research output: Contribution to journal › Article
TY - JOUR
T1 - NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina
AU - Pessanha, Miguel
AU - Turner, David L
AU - Rothery, Emma L
AU - Pankhurst, Katherine L
AU - Reid, Graeme A
AU - Chapman, Stephen K
AU - Xavier, Antonio V
AU - Salgueiro, Carlos A
PY - 2003/12/3
Y1 - 2003/12/3
N2 - Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.
AB - Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.
KW - ENERGY TRANSDUCTION
KW - paramagnetic shifts
KW - DESULFOVIBRIO-VULGARIS
KW - multihaem
KW - RESPIRATION
KW - Shewanella
KW - electron tranfer protein
KW - PUTREFACIENS MR-1
KW - ELECTRON-TRANSFER
KW - COOPERATIVITY
KW - flavocytochrome
KW - NCIMB400
KW - NMR
KW - FUMARATE REDUCTASE
KW - CYTOCHROME C(3)
KW - HEME CORE
U2 - 10.1016/S0020-1693(03)00179-8
DO - 10.1016/S0020-1693(03)00179-8
M3 - Article
VL - 356
SP - 379
EP - 381
JO - Inorganica Chimica Acta
JF - Inorganica Chimica Acta
SN - 0020-1693
ER -