NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina

Miguel Pessanha, David L Turner, Emma L Rothery, Katherine L Pankhurst, Graeme A Reid, Stephen K Chapman, Antonio V Xavier, Carlos A Salgueiro

    Research output: Contribution to journalArticle

    Abstract

    Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)379-381
    Number of pages3
    JournalInorganica Chimica Acta
    Volume356
    DOIs
    Publication statusPublished - 3 Dec 2003

    Keywords

    • ENERGY TRANSDUCTION
    • paramagnetic shifts
    • DESULFOVIBRIO-VULGARIS
    • multihaem
    • RESPIRATION
    • Shewanella
    • electron tranfer protein
    • PUTREFACIENS MR-1
    • ELECTRON-TRANSFER
    • COOPERATIVITY
    • flavocytochrome
    • NCIMB400
    • NMR
    • FUMARATE REDUCTASE
    • CYTOCHROME C(3)
    • HEME CORE

    Cite this

    Pessanha, M., Turner, D. L., Rothery, E. L., Pankhurst, K. L., Reid, G. A., Chapman, S. K., ... Salgueiro, C. A. (2003). NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina. Inorganica Chimica Acta, 356, 379-381. https://doi.org/10.1016/S0020-1693(03)00179-8
    Pessanha, Miguel ; Turner, David L ; Rothery, Emma L ; Pankhurst, Katherine L ; Reid, Graeme A ; Chapman, Stephen K ; Xavier, Antonio V ; Salgueiro, Carlos A. / NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina. In: Inorganica Chimica Acta. 2003 ; Vol. 356. pp. 379-381.
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    abstract = "Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.",
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    Pessanha, M, Turner, DL, Rothery, EL, Pankhurst, KL, Reid, GA, Chapman, SK, Xavier, AV & Salgueiro, CA 2003, 'NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina', Inorganica Chimica Acta, vol. 356, pp. 379-381. https://doi.org/10.1016/S0020-1693(03)00179-8

    NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina. / Pessanha, Miguel; Turner, David L; Rothery, Emma L; Pankhurst, Katherine L; Reid, Graeme A; Chapman, Stephen K; Xavier, Antonio V; Salgueiro, Carlos A.

    In: Inorganica Chimica Acta, Vol. 356, 03.12.2003, p. 379-381.

    Research output: Contribution to journalArticle

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    T1 - NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina

    AU - Pessanha, Miguel

    AU - Turner, David L

    AU - Rothery, Emma L

    AU - Pankhurst, Katherine L

    AU - Reid, Graeme A

    AU - Chapman, Stephen K

    AU - Xavier, Antonio V

    AU - Salgueiro, Carlos A

    PY - 2003/12/3

    Y1 - 2003/12/3

    N2 - Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.

    AB - Flavocytochrome c(3) is a periplasmic fumarate reductase with M-r 63.8 kDa, isolated from Shewanella frigidimarina NCIMB400. NMR spectroscopy was tested for its potential to elucidate the oxidation profile of each of the four haem groups in the enzyme, using the strategy developed previously to perform the thermodynamic characterization of small tetrahaem cytochromes (FEBS Lett. 314 (1992) 155). This work shows that, despite the large size of the protein, 2D-NMR NOESY experiments can be used to obtain the network of chemical exchange connectivities, between the signals of specific haem groups in sequential oxidation stages. (C) 2003 Elsevier Science B.V. All rights reserved.

    KW - ENERGY TRANSDUCTION

    KW - paramagnetic shifts

    KW - DESULFOVIBRIO-VULGARIS

    KW - multihaem

    KW - RESPIRATION

    KW - Shewanella

    KW - electron tranfer protein

    KW - PUTREFACIENS MR-1

    KW - ELECTRON-TRANSFER

    KW - COOPERATIVITY

    KW - flavocytochrome

    KW - NCIMB400

    KW - NMR

    KW - FUMARATE REDUCTASE

    KW - CYTOCHROME C(3)

    KW - HEME CORE

    U2 - 10.1016/S0020-1693(03)00179-8

    DO - 10.1016/S0020-1693(03)00179-8

    M3 - Article

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    SP - 379

    EP - 381

    JO - Inorganica Chimica Acta

    JF - Inorganica Chimica Acta

    SN - 0020-1693

    ER -

    Pessanha M, Turner DL, Rothery EL, Pankhurst KL, Reid GA, Chapman SK et al. NMR redox studies of flavocytochrome c3 from Shewanella frigidimarina. Inorganica Chimica Acta. 2003 Dec 3;356:379-381. https://doi.org/10.1016/S0020-1693(03)00179-8