Nitrofuran reductase activity in nitrofurantoin-resistant strains of Escherichia coli K12: Some with chromosomally determined resistance and others carrying R-plasmids

A S Breeze, E E Obaseiki-Ebor

    Research output: Contribution to journalArticle

    Abstract

    The nitrofuran reductase activity of Escherichia coli K12 strains was separated into two components by passage through Sepharose 4B-200. The components differed in their sensitivity to 2 M urea and their reactivity with NADH and NADPH. Some nitrofurantoin-resistant mutants had lost one component (a) while retaining the other (ß). Other mutants, resistant to higher levels of nitrofurantoin, had lost most of the ß component as well. Transconjugants which had received plasmids conferring nitrofurantoin resistance were found to have only the a component and it was markedly less active when NADH rather than NADPH was supplied as co-enzyme.

    Original languageEnglish
    Pages (from-to)543-547
    Number of pages5
    JournalJournal of Antimicrobial Chemotherapy
    Volume12
    Issue number6
    Publication statusPublished - 1983

    Fingerprint

    nitrofurans
    Escherichia coli K12
    plasmids
    NAD (coenzyme)
    NADP (coenzyme)
    mutants
    coenzymes
    agarose
    urea
    nitrofurantoin

    Cite this

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    abstract = "The nitrofuran reductase activity of Escherichia coli K12 strains was separated into two components by passage through Sepharose 4B-200. The components differed in their sensitivity to 2 M urea and their reactivity with NADH and NADPH. Some nitrofurantoin-resistant mutants had lost one component (a) while retaining the other ({\ss}). Other mutants, resistant to higher levels of nitrofurantoin, had lost most of the {\ss} component as well. Transconjugants which had received plasmids conferring nitrofurantoin resistance were found to have only the a component and it was markedly less active when NADH rather than NADPH was supplied as co-enzyme.",
    author = "Breeze, {A S} and Obaseiki-Ebor, {E E}",
    year = "1983",
    language = "English",
    volume = "12",
    pages = "543--547",
    journal = "Journal of Antimicrobial Chemotherapy",
    issn = "0305-7453",
    publisher = "Oxford University Press",
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    T1 - Nitrofuran reductase activity in nitrofurantoin-resistant strains of Escherichia coli K12

    T2 - Some with chromosomally determined resistance and others carrying R-plasmids

    AU - Breeze, A S

    AU - Obaseiki-Ebor, E E

    PY - 1983

    Y1 - 1983

    N2 - The nitrofuran reductase activity of Escherichia coli K12 strains was separated into two components by passage through Sepharose 4B-200. The components differed in their sensitivity to 2 M urea and their reactivity with NADH and NADPH. Some nitrofurantoin-resistant mutants had lost one component (a) while retaining the other (ß). Other mutants, resistant to higher levels of nitrofurantoin, had lost most of the ß component as well. Transconjugants which had received plasmids conferring nitrofurantoin resistance were found to have only the a component and it was markedly less active when NADH rather than NADPH was supplied as co-enzyme.

    AB - The nitrofuran reductase activity of Escherichia coli K12 strains was separated into two components by passage through Sepharose 4B-200. The components differed in their sensitivity to 2 M urea and their reactivity with NADH and NADPH. Some nitrofurantoin-resistant mutants had lost one component (a) while retaining the other (ß). Other mutants, resistant to higher levels of nitrofurantoin, had lost most of the ß component as well. Transconjugants which had received plasmids conferring nitrofurantoin resistance were found to have only the a component and it was markedly less active when NADH rather than NADPH was supplied as co-enzyme.

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    M3 - Article

    VL - 12

    SP - 543

    EP - 547

    JO - Journal of Antimicrobial Chemotherapy

    JF - Journal of Antimicrobial Chemotherapy

    SN - 0305-7453

    IS - 6

    ER -