Neutral Red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels – Comparing microscope and microplate methods

M. A. Miller, C. Bankier, Majed Al-Shaeri, Mark G J Hartl

Research output: Contribution to journalArticle

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Abstract

The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500 μg L− 1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method.
Original languageEnglish
Pages (from-to)903-907
Number of pages5
JournalMarine Pollution Bulletin
Volume101
Issue number2
Early online date6 Nov 2015
DOIs
Publication statusPublished - 30 Dec 2015

Keywords

  • Neutral red
  • Carbon nanotubes
  • Mytilus
  • Ecotoxicity
  • Cytotoxicity

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