Neuroprotective effects of digested polyphenols from wild blackberry species

L.R. Tavares, I. Figueira, G.J. McDougall, H.L.A. Vieira, D. Stewart, P.M. Alves, R.B. Ferreira, C.N. Santos

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    46 Citations (Scopus)

    Abstract

    Purpose
    Blackberry ingestion has been demonstrated to attenuate brain degenerative processes with the benefits ascribed to the (poly)phenolic components. The aim of this work was to evaluate the neuroprotective potential of two wild blackberry species in a neurodegeneration cell model and compare them with a commercial variety.
    Methods
    This work encompasses chemical characterization before and after an in vitro digestion and the assessment of neuroprotection by digested metabolites. Some studies targeting redox/cell death systems were also performed to assess possible neuroprotective molecular mechanisms.
    Results
    The three blackberry extracts presented some quantitative differences in polyphenol composition that could be responsible for the different responses in the neurodegeneration cell model. Commercial blackberry extracts were ineffective but both wild blackberries, Rubus brigantinus and Rubus vagabundus, presented neuroprotective effects. It was verified that a diminishment of intracellular ROS levels, modulation of glutathione levels and activation of caspases occurred during treatment. The last effect suggests a preconditioning effect since caspase activation was not accompanied by diminution in cell death and loss of functionality.
    Conclusions
    This is the first time that metabolites obtained from an in vitro digested food matrix, and tested at levels approaching the concentrations found in human plasma, have been described as inducing an adaptative response.
    © Springer-Verlag
    Original languageEnglish
    Pages (from-to)225-236
    JournalEuropean Journal of Nutrition
    Volume52
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Keywords

    • caspase activity
    • glutathione balance
    • In vitro digestion model
    • Neurodegenerative diseases
    • wild blackberries

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