Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastronintestinal digestion

L.R. Tavares; I. Figueira; D. Macedo; G.J. McDougall; M.C. Leitao; H.L.A. Vieira; D. Stewart; P.M. Alves; R.B. Ferreira; C.N. Santos

doi:10.1016/j.foodchem.2011.10.025

Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastronintestinal digestion

L.R. Tavares, I. Figueira, D. Macedo, G.J. McDougall, M.C. Leitao, H.L.A. Vieira, D. Stewart, P.M. Alves, R.B. Ferreira, C.N. Santos

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Abstract

Blackberry ingestion has been demonstrated to attenuate brain degenerative processes in rodents with the benefits ascribed to the (poly)phenolic components. The aim of this work was to assess the efficacy of blackberry polyphenolics in a neurodegeneration cell model before and after simulated gastrointestinal digestion.

Digested blackberry metabolites protected neuroblastoma cells from H2O2-induced death at low, non-toxic levels that approach physiologically-relevant serum concentrations. However, the original extracts were not protective even at fivefold higher concentrations. This potentiation may reflect alterations in the polyphenolic composition caused by the digestion procedure, as detected by liquid-chromatography-mass spectrometric analysis. This protection was not caused by modulation of the intracellular antioxidant capacity or through alteration of glutathione levels, although the original extract influenced both of these parameters. This work reinforces the importance of evaluating digested metabolites in disease cell models and highlights the possible involvement of other mechanisms beyond antioxidant systems.
Copyright © 2012, Elsevier

Original language	English
Pages (from-to)	1443-1452
Number of pages	10
Journal	Food Chemistry
Volume	131
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2011.10.025
Publication status	Published - 15 Apr 2012

Keywords

blackberry
In vitro digestion model
Neurodegenerative diseases
Phenolic compounds

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10.1016/j.foodchem.2011.10.025

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title = "Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastronintestinal digestion",

abstract = "Blackberry ingestion has been demonstrated to attenuate brain degenerative processes in rodents with the benefits ascribed to the (poly)phenolic components. The aim of this work was to assess the efficacy of blackberry polyphenolics in a neurodegeneration cell model before and after simulated gastrointestinal digestion.Digested blackberry metabolites protected neuroblastoma cells from H2O2-induced death at low, non-toxic levels that approach physiologically-relevant serum concentrations. However, the original extracts were not protective even at fivefold higher concentrations. This potentiation may reflect alterations in the polyphenolic composition caused by the digestion procedure, as detected by liquid-chromatography-mass spectrometric analysis. This protection was not caused by modulation of the intracellular antioxidant capacity or through alteration of glutathione levels, although the original extract influenced both of these parameters. This work reinforces the importance of evaluating digested metabolites in disease cell models and highlights the possible involvement of other mechanisms beyond antioxidant systems.Copyright {\textcopyright} 2012, Elsevier",

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author = "L.R. Tavares and I. Figueira and D. Macedo and G.J. McDougall and M.C. Leitao and H.L.A. Vieira and D. Stewart and P.M. Alves and R.B. Ferreira and C.N. Santos",

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T1 - Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastronintestinal digestion

AU - Tavares, L.R.

AU - Figueira, I.

AU - Macedo, D.

AU - McDougall, G.J.

AU - Leitao, M.C.

AU - Vieira, H.L.A.

AU - Stewart, D.

AU - Alves, P.M.

AU - Ferreira, R.B.

AU - Santos, C.N.

PY - 2012/4/15

Y1 - 2012/4/15

N2 - Blackberry ingestion has been demonstrated to attenuate brain degenerative processes in rodents with the benefits ascribed to the (poly)phenolic components. The aim of this work was to assess the efficacy of blackberry polyphenolics in a neurodegeneration cell model before and after simulated gastrointestinal digestion.Digested blackberry metabolites protected neuroblastoma cells from H2O2-induced death at low, non-toxic levels that approach physiologically-relevant serum concentrations. However, the original extracts were not protective even at fivefold higher concentrations. This potentiation may reflect alterations in the polyphenolic composition caused by the digestion procedure, as detected by liquid-chromatography-mass spectrometric analysis. This protection was not caused by modulation of the intracellular antioxidant capacity or through alteration of glutathione levels, although the original extract influenced both of these parameters. This work reinforces the importance of evaluating digested metabolites in disease cell models and highlights the possible involvement of other mechanisms beyond antioxidant systems.Copyright © 2012, Elsevier

AB - Blackberry ingestion has been demonstrated to attenuate brain degenerative processes in rodents with the benefits ascribed to the (poly)phenolic components. The aim of this work was to assess the efficacy of blackberry polyphenolics in a neurodegeneration cell model before and after simulated gastrointestinal digestion.Digested blackberry metabolites protected neuroblastoma cells from H2O2-induced death at low, non-toxic levels that approach physiologically-relevant serum concentrations. However, the original extracts were not protective even at fivefold higher concentrations. This potentiation may reflect alterations in the polyphenolic composition caused by the digestion procedure, as detected by liquid-chromatography-mass spectrometric analysis. This protection was not caused by modulation of the intracellular antioxidant capacity or through alteration of glutathione levels, although the original extract influenced both of these parameters. This work reinforces the importance of evaluating digested metabolites in disease cell models and highlights the possible involvement of other mechanisms beyond antioxidant systems.Copyright © 2012, Elsevier

KW - blackberry

KW - In vitro digestion model

KW - Neurodegenerative diseases

KW - Phenolic compounds

U2 - 10.1016/j.foodchem.2011.10.025

DO - 10.1016/j.foodchem.2011.10.025

M3 - Article

VL - 131

SP - 1443

EP - 1452

JO - Food Chemistry

JF - Food Chemistry

IS - 4

ER -

Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastronintestinal digestion

Abstract

Keywords

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