Nanotopology potentiates growth hormone signalling and osteogenesis of mesenchymal stem cells

Jessie R. Wang, S. Faisal Ahmed, Nikolaj Gadegaard, R. M. Dominic Meek, Matthew J. Dalby, Stephen J Yarwood

    Research output: Contribution to journalArticle

    Abstract

    Custom engineered materials can influence the differentiation of human mesenchymal stem cells (MSCs) towards osteoblasts, chondrocytes and adipocytes, through the control of chemistry, stiffness and nanoscale topography. Here we demonstrate that polycaprolactone growth surfaces engineered with disordered (but controlled) 120 nm diameter dots (NSQ50), but not flat surfaces, promote osteogenic conversion of MSCs in the absence of other osteogenic stimuli. Differentiating MSCs on NSQ50 were found to express growth hormone receptors (GH) and stimulation with recombinant human GH (rhGH) further enhanced NSQ50-driven osteogenic conversion of MSCs. This increased osteogenesis coincided with an enhanced ability of GH to activate ERK MAP kinase on NSQ50, but not on flat topology. The importance of ERK for MSC differentiation was demonstrated by using the inhibitor of ERK activation, U0126, which completely suppressed osteogenesis of GH-stimulated MSCs on NSQ50. The ability of GH to activate ERK in MSCs may therefore be a central control mechanism underlying bone development and growth.

    Original languageEnglish
    Pages (from-to)245-250
    Number of pages6
    JournalGrowth Hormone and IGF Research
    Volume24
    Issue number6
    DOIs
    Publication statusPublished - Dec 2014

    Keywords

    • Alkaline Phosphatase
    • Blotting, Western
    • Cell Differentiation
    • Cells, Cultured
    • Extracellular Signal-Regulated MAP Kinases
    • Human Growth Hormone
    • Humans
    • Immunoenzyme Techniques
    • Mesenchymal Stromal Cells
    • Nanotechnology
    • Osteoblasts
    • Osteogenesis
    • Polyesters
    • RNA, Messenger
    • Real-Time Polymerase Chain Reaction
    • Reverse Transcriptase Polymerase Chain Reaction
    • Signal Transduction

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