Mutagenesis using Tn917 in Bacillus licheniformis

M. R. Wati, F. G. Priest, W. J. Mitchell

Research output: Contribution to journalArticle

Abstract

Two plasmid vectors containing Tn917 have been transformed into protoplasts of Bacillus licheniformis. Replication of the vectors was inhibited at high temperature. The transposon integrated efficiently into the chromosome and auxotroph formation was high (9% of transposed colonies). Most of these were argG or argH indicating a hot-spot region for integration of Tn917 into the chromosome. However, about 0.5% of the mutants had other auxotrophies.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalFEMS Microbiology Letters
Volume71
Issue number1-2
Publication statusPublished - 1990

Keywords

  • arg mutants
  • argininosuccinate lyase
  • Bacillus licheniformis
  • transposon mutagenesis

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