Two plasmid vectors containing Tn917 have been transformed into protoplasts of Bacillus licheniformis. Replication of the vectors was inhibited at high temperature. The transposon integrated efficiently into the chromosome and auxotroph formation was high (9% of transposed colonies). Most of these were argG or argH indicating a hot-spot region for integration of Tn917 into the chromosome. However, about 0.5% of the mutants had other auxotrophies.
|Number of pages||4|
|Journal||FEMS Microbiology Letters|
|Publication status||Published - 1990|
- arg mutants
- argininosuccinate lyase
- Bacillus licheniformis
- transposon mutagenesis