Munc 18-1 Protein Molecules Move between Membrane Molecular Depots Distinct from Vesicle Docking Sites

Annya M. Smyth, Lei Yang, Kirsty J. Martin, Charlotte Hamilton, Weiping Lu, Michael A. Cousin, Colin Rickman, Rory R. Duncan

Research output: Contribution to journalArticle

10 Citations (Scopus)
152 Downloads (Pure)

Abstract

Four evolutionarily conserved proteins are required for mammalian regulated exocytosis: three SNARE proteins, syntaxin, SNAP-25, and synaptobrevin, and the SM protein, Munc18-1. Here, using single-molecule imaging, we measured the spatial distribution of large cohorts of single Munc18-1 molecules correlated with the positions of single secretory vesicles in a functionally rescued Munc18-1-null cellular model. Munc18-1 molecules were nonrandomly distributed across the plasma membrane in a manner not directed by mode of interaction with syntaxin1, with a small mean number of molecules observed to reside under membrane resident vesicles. Surprisingly, we found that the majority of vesicles in fully secretion-competent cells had no Munc18-1 associated within distances relevant to plasma membrane-vesicle SNARE interactions. Live cell imaging of Munc18-1 molecule dynamics revealed that the density of Munc18-1 molecules at the plasma membrane anticorrelated with molecular speed, with single Munc18-1 molecules displaying directed motion between membrane hotspots enriched in syntaxin1a. Our findings demonstrate that Munc18-1 molecules move between membrane depots distinct from vesicle morphological docking sites.

Original languageEnglish
Pages (from-to)5102-5113
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number7
Early online date6 Dec 2012
DOIs
Publication statusPublished - 15 Feb 2013

Keywords

  • LOCALIZATION MICROSCOPY
  • EXOCYTOSIS
  • SECRETORY GRANULES
  • BINDING
  • FUSION
  • CONFORMATIONAL SWITCH
  • NEURONAL SNARE COMPLEX
  • SYNTAXIN CLUSTERS
  • LIVING CELLS
  • LIVE CELLS

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