Multiplexed fibre optic sensing in the distal lung (Conference Presentation)

Tushar R. Choudhary, Michael George Tanner, Alicia Megia-Fernandez, Kerrianne Harrington, Harry A. Wood, Sunay Chankeshwara, Patricia Zhu, Debaditya Choudhury, Fei Yu, Robert R. Thomson, Rory R. Duncan, Kevin Dhaliwal, Mark Bradley

Research output: Contribution to conferenceOther

Abstract

We present a toolkit for a multiplexed pH and oxygen sensing probe in the distal lung using multicore fibres. Measuring physiological relevant parameters like pH and oxygen is of significant importance in understanding changes associated with disease pathology. We present here, a single multicore fibre based pH and oxygen sensing probe which can be used with a standard bronchoscope to perform in vivo measurements in the distal lung. The multiplexed probe consists of fluorescent pH sensors (fluorescein based) and oxygen sensors (Palladium porphyrin complex based) covalently bonded to silica microspheres (10 µm) loaded on the distal facet of a 19 core (10 µm core diameter) multicore fibre (total diameter of ~150 µm excluding coating). Pits are formed by selectively etching the cores using hydrofluoric acid, multiplexing is achieved through the self-location of individual probes on differing cores. This architecture can be expanded to include probes for further parameters. Robust measurements are demonstrated of self-referencing fluorophores, not limited by photobleaching, with short (100ms) measurement times at low (~10µW) illumination powers. We have performed on bench calibration and tests of in vitro tissue models and in an ovine whole lung model to validate our sensors. The pH sensor is demonstrated in the physiologically relevant range of pH 5 to pH 8.5 and with an accuracy of ± 0.05 pH units. The oxygen sensor is demonstrated in gas mixtures downwards from 20% oxygen and in liquid saturated with 20% oxygen mixtures (~8mg/L) down to full depletion (0mg/L) with ~0.5mg/L accuracy.
Original languageEnglish
Pages100580E
DOIs
Publication statusPublished - 19 Apr 2017
EventSPIE BiOS 2017 - San Fransisco, United States
Duration: 28 Jan 20172 Feb 2017

Conference

ConferenceSPIE BiOS 2017
CountryUnited States
CitySan Fransisco
Period28/01/172/02/17

Fingerprint

Fiber optics
Oxygen
pH sensors
Oxygen sensors
Fibers
Hydrofluoric Acid
Photobleaching
Fluorophores
Porphyrins
Palladium
Pathology
Time measurement
Fluorescein
Microspheres
Fluorescent Dyes
Multiplexing
Gas mixtures
Silicon Dioxide
Etching
Lighting

Cite this

Choudhary, T. R., Tanner, M. G., Megia-Fernandez, A., Harrington, K., Wood, H. A., Chankeshwara, S., ... Bradley, M. (2017). Multiplexed fibre optic sensing in the distal lung (Conference Presentation). 100580E. SPIE BiOS 2017, San Fransisco, United States. https://doi.org/10.1117/12.2249982
Choudhary, Tushar R. ; Tanner, Michael George ; Megia-Fernandez, Alicia ; Harrington, Kerrianne ; Wood, Harry A. ; Chankeshwara, Sunay ; Zhu, Patricia ; Choudhury, Debaditya ; Yu, Fei ; Thomson, Robert R. ; Duncan, Rory R. ; Dhaliwal, Kevin ; Bradley, Mark. / Multiplexed fibre optic sensing in the distal lung (Conference Presentation). SPIE BiOS 2017, San Fransisco, United States.
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abstract = "We present a toolkit for a multiplexed pH and oxygen sensing probe in the distal lung using multicore fibres. Measuring physiological relevant parameters like pH and oxygen is of significant importance in understanding changes associated with disease pathology. We present here, a single multicore fibre based pH and oxygen sensing probe which can be used with a standard bronchoscope to perform in vivo measurements in the distal lung. The multiplexed probe consists of fluorescent pH sensors (fluorescein based) and oxygen sensors (Palladium porphyrin complex based) covalently bonded to silica microspheres (10 µm) loaded on the distal facet of a 19 core (10 µm core diameter) multicore fibre (total diameter of ~150 µm excluding coating). Pits are formed by selectively etching the cores using hydrofluoric acid, multiplexing is achieved through the self-location of individual probes on differing cores. This architecture can be expanded to include probes for further parameters. Robust measurements are demonstrated of self-referencing fluorophores, not limited by photobleaching, with short (100ms) measurement times at low (~10µW) illumination powers. We have performed on bench calibration and tests of in vitro tissue models and in an ovine whole lung model to validate our sensors. The pH sensor is demonstrated in the physiologically relevant range of pH 5 to pH 8.5 and with an accuracy of ± 0.05 pH units. The oxygen sensor is demonstrated in gas mixtures downwards from 20{\%} oxygen and in liquid saturated with 20{\%} oxygen mixtures (~8mg/L) down to full depletion (0mg/L) with ~0.5mg/L accuracy.",
author = "Choudhary, {Tushar R.} and Tanner, {Michael George} and Alicia Megia-Fernandez and Kerrianne Harrington and Wood, {Harry A.} and Sunay Chankeshwara and Patricia Zhu and Debaditya Choudhury and Fei Yu and Thomson, {Robert R.} and Duncan, {Rory R.} and Kevin Dhaliwal and Mark Bradley",
note = "Presentation only; SPIE BiOS 2017 ; Conference date: 28-01-2017 Through 02-02-2017",
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Choudhary, TR, Tanner, MG, Megia-Fernandez, A, Harrington, K, Wood, HA, Chankeshwara, S, Zhu, P, Choudhury, D, Yu, F, Thomson, RR, Duncan, RR, Dhaliwal, K & Bradley, M 2017, 'Multiplexed fibre optic sensing in the distal lung (Conference Presentation)' SPIE BiOS 2017, San Fransisco, United States, 28/01/17 - 2/02/17, pp. 100580E. https://doi.org/10.1117/12.2249982

Multiplexed fibre optic sensing in the distal lung (Conference Presentation). / Choudhary, Tushar R.; Tanner, Michael George; Megia-Fernandez, Alicia; Harrington, Kerrianne; Wood, Harry A.; Chankeshwara, Sunay; Zhu, Patricia; Choudhury, Debaditya; Yu, Fei; Thomson, Robert R.; Duncan, Rory R.; Dhaliwal, Kevin; Bradley, Mark.

2017. 100580E SPIE BiOS 2017, San Fransisco, United States.

Research output: Contribution to conferenceOther

TY - CONF

T1 - Multiplexed fibre optic sensing in the distal lung (Conference Presentation)

AU - Choudhary, Tushar R.

AU - Tanner, Michael George

AU - Megia-Fernandez, Alicia

AU - Harrington, Kerrianne

AU - Wood, Harry A.

AU - Chankeshwara, Sunay

AU - Zhu, Patricia

AU - Choudhury, Debaditya

AU - Yu, Fei

AU - Thomson, Robert R.

AU - Duncan, Rory R.

AU - Dhaliwal, Kevin

AU - Bradley, Mark

N1 - Presentation only

PY - 2017/4/19

Y1 - 2017/4/19

N2 - We present a toolkit for a multiplexed pH and oxygen sensing probe in the distal lung using multicore fibres. Measuring physiological relevant parameters like pH and oxygen is of significant importance in understanding changes associated with disease pathology. We present here, a single multicore fibre based pH and oxygen sensing probe which can be used with a standard bronchoscope to perform in vivo measurements in the distal lung. The multiplexed probe consists of fluorescent pH sensors (fluorescein based) and oxygen sensors (Palladium porphyrin complex based) covalently bonded to silica microspheres (10 µm) loaded on the distal facet of a 19 core (10 µm core diameter) multicore fibre (total diameter of ~150 µm excluding coating). Pits are formed by selectively etching the cores using hydrofluoric acid, multiplexing is achieved through the self-location of individual probes on differing cores. This architecture can be expanded to include probes for further parameters. Robust measurements are demonstrated of self-referencing fluorophores, not limited by photobleaching, with short (100ms) measurement times at low (~10µW) illumination powers. We have performed on bench calibration and tests of in vitro tissue models and in an ovine whole lung model to validate our sensors. The pH sensor is demonstrated in the physiologically relevant range of pH 5 to pH 8.5 and with an accuracy of ± 0.05 pH units. The oxygen sensor is demonstrated in gas mixtures downwards from 20% oxygen and in liquid saturated with 20% oxygen mixtures (~8mg/L) down to full depletion (0mg/L) with ~0.5mg/L accuracy.

AB - We present a toolkit for a multiplexed pH and oxygen sensing probe in the distal lung using multicore fibres. Measuring physiological relevant parameters like pH and oxygen is of significant importance in understanding changes associated with disease pathology. We present here, a single multicore fibre based pH and oxygen sensing probe which can be used with a standard bronchoscope to perform in vivo measurements in the distal lung. The multiplexed probe consists of fluorescent pH sensors (fluorescein based) and oxygen sensors (Palladium porphyrin complex based) covalently bonded to silica microspheres (10 µm) loaded on the distal facet of a 19 core (10 µm core diameter) multicore fibre (total diameter of ~150 µm excluding coating). Pits are formed by selectively etching the cores using hydrofluoric acid, multiplexing is achieved through the self-location of individual probes on differing cores. This architecture can be expanded to include probes for further parameters. Robust measurements are demonstrated of self-referencing fluorophores, not limited by photobleaching, with short (100ms) measurement times at low (~10µW) illumination powers. We have performed on bench calibration and tests of in vitro tissue models and in an ovine whole lung model to validate our sensors. The pH sensor is demonstrated in the physiologically relevant range of pH 5 to pH 8.5 and with an accuracy of ± 0.05 pH units. The oxygen sensor is demonstrated in gas mixtures downwards from 20% oxygen and in liquid saturated with 20% oxygen mixtures (~8mg/L) down to full depletion (0mg/L) with ~0.5mg/L accuracy.

U2 - 10.1117/12.2249982

DO - 10.1117/12.2249982

M3 - Other

SP - 100580E

ER -

Choudhary TR, Tanner MG, Megia-Fernandez A, Harrington K, Wood HA, Chankeshwara S et al. Multiplexed fibre optic sensing in the distal lung (Conference Presentation). 2017. SPIE BiOS 2017, San Fransisco, United States. https://doi.org/10.1117/12.2249982