Monitoring polysaccharide synthesis in Candida albicans

J. A. Brown; B. J. Catley

doi:10.1016/0008-6215(92)85071-7

Monitoring polysaccharide synthesis in Candida albicans

J. A. Brown, B. J. Catley

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A method is described that measures the rate of [¹⁴C]-labelling of (1?6)-ß-D-glucan, mannoprotein, and (1?3)/(1?6)-ß-D-glucan during approximately one-tenth of the doubling time of Candida ulbicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the composition of newly constructed cell walls that might otherwise be masked by previously deposited envelope.

Original language	English
Pages (from-to)	195-202
Number of pages	8
Journal	Carbohydrate Research
Volume	227
DOIs	https://doi.org/10.1016/0008-6215(92)85071-7
Publication status	Published - 6 Apr 1992

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@article{80bbf71ab6384b8bb0f67588e0baefc7,

title = "Monitoring polysaccharide synthesis in Candida albicans",

abstract = "A method is described that measures the rate of [14C]-labelling of (1?6)-{\ss}-D-glucan, mannoprotein, and (1?3)/(1?6)-{\ss}-D-glucan during approximately one-tenth of the doubling time of Candida ulbicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the composition of newly constructed cell walls that might otherwise be masked by previously deposited envelope.",

author = "Brown, {J. A.} and Catley, {B. J.}",

year = "1992",

month = apr,

day = "6",

doi = "10.1016/0008-6215(92)85071-7",

language = "English",

volume = "227",

pages = "195--202",

journal = "Carbohydrate Research",

publisher = "Elsevier",

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T1 - Monitoring polysaccharide synthesis in Candida albicans

AU - Brown, J. A.

AU - Catley, B. J.

PY - 1992/4/6

Y1 - 1992/4/6

N2 - A method is described that measures the rate of [14C]-labelling of (1?6)-ß-D-glucan, mannoprotein, and (1?3)/(1?6)-ß-D-glucan during approximately one-tenth of the doubling time of Candida ulbicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the composition of newly constructed cell walls that might otherwise be masked by previously deposited envelope.

AB - A method is described that measures the rate of [14C]-labelling of (1?6)-ß-D-glucan, mannoprotein, and (1?3)/(1?6)-ß-D-glucan during approximately one-tenth of the doubling time of Candida ulbicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the composition of newly constructed cell walls that might otherwise be masked by previously deposited envelope.

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U2 - 10.1016/0008-6215(92)85071-7

DO - 10.1016/0008-6215(92)85071-7

M3 - Article

VL - 227

SP - 195

EP - 202

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Monitoring polysaccharide synthesis in Candida albicans

Abstract

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