TY - JOUR
T1 - Molecular typing of Bacillus thuringiensis serovars by RAPD-PCR
AU - Gaviria Rivera, Adelaida M.
AU - Priest, Fergus G.
PY - 2003/6
Y1 - 2003/6
N2 - One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and volworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars aizawai, canadensis, entomocidus and sotto biotype dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.
AB - One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and volworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars aizawai, canadensis, entomocidus and sotto biotype dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.
KW - Bacillus thuringiensis
KW - Clone
KW - RAPD
KW - Serovar
UR - http://www.scopus.com/inward/record.url?scp=0037676077&partnerID=8YFLogxK
U2 - 10.1078/072320203322346100
DO - 10.1078/072320203322346100
M3 - Article
SN - 0723-2020
VL - 26
SP - 254
EP - 261
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 2
ER -