Molecular typing of Bacillus thuringiensis serovars by RAPD-PCR

Adelaida M. Gaviria Rivera, Fergus G. Priest

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and volworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars aizawai, canadensis, entomocidus and sotto biotype dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.

    Original languageEnglish
    Pages (from-to)254-261
    Number of pages8
    JournalSystematic and Applied Microbiology
    Volume26
    Issue number2
    DOIs
    Publication statusPublished - Jun 2003

    Keywords

    • Bacillus thuringiensis
    • Clone
    • RAPD
    • Serovar

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