Molecular profile of organ culture-stored corneal epithelium

LGR5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells

Joanna Brzeszczynska, Kanna Ramaesh, Baljean Dhillon, James A Ross

    Research output: Contribution to journalArticle

    Abstract

    Long-term preservation of corneal limbal epithelium may decrease its quality and change the molecular signature of the limbal epithelial stem cells. In this study we have investigated the molecular profile of isolated corneal epithelial cells that have been in storage for an extended time. Isolated cells were characterised by the expression profile of different cytokeratins and markers of squamous metaplasia (vimentin and a-actin). Furthermore, we examined global markers of adult stem cells including p63a and ABCG2 but also LGR5 as a novel stem cell marker. Immunocytochemical staining and PCR analysis of p63a, ABCG2 and LGR5 revealed the existence of side-population cells with a stem-cell phenotype and maintenance of corneal limbal stem cell properties. LGR5 expression can be related to cellular stemness and can be considered as a new phenotypic marker of residual human corneal limbal stem cells. However, the existence of CK10 together with co-expressed a-actin and vimentin suggests that the corneas investigated were under oxidative stress and showed evidence of squamous metaplasia.
    Original languageEnglish
    Pages (from-to)871-6
    Number of pages6
    JournalInternational Journal of Molecular Medicine
    Volume29
    Issue number5
    DOIs
    Publication statusPublished - May 2012

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    Corneal Epithelium
    Organ Culture Techniques
    Stem Cells
    Epithelial Cells
    Metaplasia
    Vimentin
    Actins
    Side-Population Cells
    Adult Stem Cells
    Keratins
    Cornea
    Oxidative Stress
    Maintenance
    Staining and Labeling
    Phenotype
    Polymerase Chain Reaction

    Cite this

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    title = "Molecular profile of organ culture-stored corneal epithelium: LGR5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells",
    abstract = "Long-term preservation of corneal limbal epithelium may decrease its quality and change the molecular signature of the limbal epithelial stem cells. In this study we have investigated the molecular profile of isolated corneal epithelial cells that have been in storage for an extended time. Isolated cells were characterised by the expression profile of different cytokeratins and markers of squamous metaplasia (vimentin and a-actin). Furthermore, we examined global markers of adult stem cells including p63a and ABCG2 but also LGR5 as a novel stem cell marker. Immunocytochemical staining and PCR analysis of p63a, ABCG2 and LGR5 revealed the existence of side-population cells with a stem-cell phenotype and maintenance of corneal limbal stem cell properties. LGR5 expression can be related to cellular stemness and can be considered as a new phenotypic marker of residual human corneal limbal stem cells. However, the existence of CK10 together with co-expressed a-actin and vimentin suggests that the corneas investigated were under oxidative stress and showed evidence of squamous metaplasia.",
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    Molecular profile of organ culture-stored corneal epithelium : LGR5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. / Brzeszczynska, Joanna; Ramaesh, Kanna; Dhillon, Baljean; Ross, James A.

    In: International Journal of Molecular Medicine, Vol. 29, No. 5, 05.2012, p. 871-6.

    Research output: Contribution to journalArticle

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    AU - Brzeszczynska, Joanna

    AU - Ramaesh, Kanna

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    AU - Ross, James A

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    AB - Long-term preservation of corneal limbal epithelium may decrease its quality and change the molecular signature of the limbal epithelial stem cells. In this study we have investigated the molecular profile of isolated corneal epithelial cells that have been in storage for an extended time. Isolated cells were characterised by the expression profile of different cytokeratins and markers of squamous metaplasia (vimentin and a-actin). Furthermore, we examined global markers of adult stem cells including p63a and ABCG2 but also LGR5 as a novel stem cell marker. Immunocytochemical staining and PCR analysis of p63a, ABCG2 and LGR5 revealed the existence of side-population cells with a stem-cell phenotype and maintenance of corneal limbal stem cell properties. LGR5 expression can be related to cellular stemness and can be considered as a new phenotypic marker of residual human corneal limbal stem cells. However, the existence of CK10 together with co-expressed a-actin and vimentin suggests that the corneas investigated were under oxidative stress and showed evidence of squamous metaplasia.

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