Molecular characterization of two lipoxygenases from barley

Jan R. Van Mechelen, Robert C. Schuurink, Miriam Smits, Andreas Graner, Anneke C. Douma, N. J A Sedee, Nathalie F. Schmitt, Betty E. Valk

    Research output: Contribution to journalArticle

    Abstract

    Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and LoxB map on chromosome 4 and LoxC on chromosome 7. Two isoenzymes, LOX1 and LOX2, have been purified previously from germinating barley and characterized. LOX1 is encoded by LoxA, while LOX2 is encoded by LoxC. The product related to the third cDNA (loxB) has not been identified so far, suggesting a low protein abundance for the corresponding isoform in barley. Transcripts corresponding with these LOX genes are predominantly observed in grain and in seedling, whereas transcripts corresponding to LoxB and LoxC are also observed in mature vegetative tissue. No lipoxygenase mRNA could be detected in aleurone layer of germinating grain. No significant differences in lipoxygenase mRNA levels were observed in developing grains grown under dormant or non-dormant conditions, suggesting that LOX is not directly involved in induction of grain dormancy.

    Original languageEnglish
    Pages (from-to)1283-1298
    Number of pages16
    JournalPlant Molecular Biology
    Volume39
    Issue number6
    DOIs
    Publication statusPublished - Apr 1999

    Fingerprint

    Loxa
    lipoxygenase
    barley
    Hordeum vulgare subsp. vulgare
    chromosomes
    aleurone layer
    Southern blotting
    dormancy
    isozymes
    restriction fragment length polymorphism
    genes
    loci
    seedlings
    proteins

    Keywords

    • Barley
    • Chromosomal mapping
    • Grain dormancy
    • Hordeum distichum
    • Jasmonate (jasmonic acid)
    • Lipoxygenase (LOX)

    Cite this

    Van Mechelen, J. R., Schuurink, R. C., Smits, M., Graner, A., Douma, A. C., Sedee, N. J. A., ... Valk, B. E. (1999). Molecular characterization of two lipoxygenases from barley. Plant Molecular Biology, 39(6), 1283-1298. https://doi.org/10.1023/A:1006118003998
    Van Mechelen, Jan R. ; Schuurink, Robert C. ; Smits, Miriam ; Graner, Andreas ; Douma, Anneke C. ; Sedee, N. J A ; Schmitt, Nathalie F. ; Valk, Betty E. / Molecular characterization of two lipoxygenases from barley. In: Plant Molecular Biology. 1999 ; Vol. 39, No. 6. pp. 1283-1298.
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    abstract = "Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and LoxB map on chromosome 4 and LoxC on chromosome 7. Two isoenzymes, LOX1 and LOX2, have been purified previously from germinating barley and characterized. LOX1 is encoded by LoxA, while LOX2 is encoded by LoxC. The product related to the third cDNA (loxB) has not been identified so far, suggesting a low protein abundance for the corresponding isoform in barley. Transcripts corresponding with these LOX genes are predominantly observed in grain and in seedling, whereas transcripts corresponding to LoxB and LoxC are also observed in mature vegetative tissue. No lipoxygenase mRNA could be detected in aleurone layer of germinating grain. No significant differences in lipoxygenase mRNA levels were observed in developing grains grown under dormant or non-dormant conditions, suggesting that LOX is not directly involved in induction of grain dormancy.",
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    Van Mechelen, JR, Schuurink, RC, Smits, M, Graner, A, Douma, AC, Sedee, NJA, Schmitt, NF & Valk, BE 1999, 'Molecular characterization of two lipoxygenases from barley', Plant Molecular Biology, vol. 39, no. 6, pp. 1283-1298. https://doi.org/10.1023/A:1006118003998

    Molecular characterization of two lipoxygenases from barley. / Van Mechelen, Jan R.; Schuurink, Robert C.; Smits, Miriam; Graner, Andreas; Douma, Anneke C.; Sedee, N. J A; Schmitt, Nathalie F.; Valk, Betty E.

    In: Plant Molecular Biology, Vol. 39, No. 6, 04.1999, p. 1283-1298.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Molecular characterization of two lipoxygenases from barley

    AU - Van Mechelen, Jan R.

    AU - Schuurink, Robert C.

    AU - Smits, Miriam

    AU - Graner, Andreas

    AU - Douma, Anneke C.

    AU - Sedee, N. J A

    AU - Schmitt, Nathalie F.

    AU - Valk, Betty E.

    PY - 1999/4

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    N2 - Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and LoxB map on chromosome 4 and LoxC on chromosome 7. Two isoenzymes, LOX1 and LOX2, have been purified previously from germinating barley and characterized. LOX1 is encoded by LoxA, while LOX2 is encoded by LoxC. The product related to the third cDNA (loxB) has not been identified so far, suggesting a low protein abundance for the corresponding isoform in barley. Transcripts corresponding with these LOX genes are predominantly observed in grain and in seedling, whereas transcripts corresponding to LoxB and LoxC are also observed in mature vegetative tissue. No lipoxygenase mRNA could be detected in aleurone layer of germinating grain. No significant differences in lipoxygenase mRNA levels were observed in developing grains grown under dormant or non-dormant conditions, suggesting that LOX is not directly involved in induction of grain dormancy.

    AB - Two full-length lipoxygenase cDNA sequences (LoxB and LoxC) from barley (Hordeum distichum cv. L. Triumph) are described. The cDNAs share high homology with the barley LoxA cDNA. Southern blotting experiments indicate single copy numbers of the three lipoxygenase genes. RFLP mapping revealed the presence of single lipoxygenase loci. LoxA and LoxB map on chromosome 4 and LoxC on chromosome 7. Two isoenzymes, LOX1 and LOX2, have been purified previously from germinating barley and characterized. LOX1 is encoded by LoxA, while LOX2 is encoded by LoxC. The product related to the third cDNA (loxB) has not been identified so far, suggesting a low protein abundance for the corresponding isoform in barley. Transcripts corresponding with these LOX genes are predominantly observed in grain and in seedling, whereas transcripts corresponding to LoxB and LoxC are also observed in mature vegetative tissue. No lipoxygenase mRNA could be detected in aleurone layer of germinating grain. No significant differences in lipoxygenase mRNA levels were observed in developing grains grown under dormant or non-dormant conditions, suggesting that LOX is not directly involved in induction of grain dormancy.

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    KW - Chromosomal mapping

    KW - Grain dormancy

    KW - Hordeum distichum

    KW - Jasmonate (jasmonic acid)

    KW - Lipoxygenase (LOX)

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    Van Mechelen JR, Schuurink RC, Smits M, Graner A, Douma AC, Sedee NJA et al. Molecular characterization of two lipoxygenases from barley. Plant Molecular Biology. 1999 Apr;39(6):1283-1298. https://doi.org/10.1023/A:1006118003998