Molecular characterization of plasmid-mediated oxytetracycline resistance in Aeromonas salmonicida

C. A. Adams, B. Austin, P. G. Meaden, D. McIntosh

    Research output: Contribution to journalArticle

    Abstract

    Using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (OT)-resistant isolates of Aeromonas salmonicida transferred the OT resistance phenotype to Escherichia coli. The OT resistance phenotype was encoded by high-molecular-weight R-plasmids that were capable of transferring OT resistance to both environmental and clinical isolates of Aeromonas spp. The molecular basis for antibiotic resistance in OT-resistant isolates of A. salmonicida was determined. The OT resistance determinant from one plasmid (pASOT) of A. salmonicida was cloned and used in Southern blotting and hybridization experiments as a probe. The determinant was identified on a 5.4-kb EcoRI fragment on R-plasmids from the 19 OT-resistant isolates of A. salmonicida. Hybridization with plasmids encoding the five classes (classes A to E) of OT resistance determinants demonstrated that the OT resistance plasmids of the 19 A. salmonicida isolates carried the class A resistance determinant. Analysis of data generated from restriction enzyme digests showed that the OT resistance plasmids were not identical; three profiles were characterized, two of which showed a high degree of homology.

    Original languageEnglish
    Pages (from-to)4194-4201
    Number of pages8
    JournalApplied and Environmental Microbiology
    Volume64
    Issue number11
    Publication statusPublished - Nov 1998

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    Aeromonas salmonicida
    oxytetracycline
    plasmids
    Southern blotting
    phenotype
    Aeromonas
    antibiotic resistance
    data analysis
    hybridization
    molecular weight
    Escherichia coli

    Cite this

    Adams, C. A. ; Austin, B. ; Meaden, P. G. ; McIntosh, D. / Molecular characterization of plasmid-mediated oxytetracycline resistance in Aeromonas salmonicida. In: Applied and Environmental Microbiology. 1998 ; Vol. 64, No. 11. pp. 4194-4201.
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    abstract = "Using broth conjugation, we found that 19 of 29 (66{\%}) oxytetracycline (OT)-resistant isolates of Aeromonas salmonicida transferred the OT resistance phenotype to Escherichia coli. The OT resistance phenotype was encoded by high-molecular-weight R-plasmids that were capable of transferring OT resistance to both environmental and clinical isolates of Aeromonas spp. The molecular basis for antibiotic resistance in OT-resistant isolates of A. salmonicida was determined. The OT resistance determinant from one plasmid (pASOT) of A. salmonicida was cloned and used in Southern blotting and hybridization experiments as a probe. The determinant was identified on a 5.4-kb EcoRI fragment on R-plasmids from the 19 OT-resistant isolates of A. salmonicida. Hybridization with plasmids encoding the five classes (classes A to E) of OT resistance determinants demonstrated that the OT resistance plasmids of the 19 A. salmonicida isolates carried the class A resistance determinant. Analysis of data generated from restriction enzyme digests showed that the OT resistance plasmids were not identical; three profiles were characterized, two of which showed a high degree of homology.",
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    Molecular characterization of plasmid-mediated oxytetracycline resistance in Aeromonas salmonicida. / Adams, C. A.; Austin, B.; Meaden, P. G.; McIntosh, D.

    In: Applied and Environmental Microbiology, Vol. 64, No. 11, 11.1998, p. 4194-4201.

    Research output: Contribution to journalArticle

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    AB - Using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (OT)-resistant isolates of Aeromonas salmonicida transferred the OT resistance phenotype to Escherichia coli. The OT resistance phenotype was encoded by high-molecular-weight R-plasmids that were capable of transferring OT resistance to both environmental and clinical isolates of Aeromonas spp. The molecular basis for antibiotic resistance in OT-resistant isolates of A. salmonicida was determined. The OT resistance determinant from one plasmid (pASOT) of A. salmonicida was cloned and used in Southern blotting and hybridization experiments as a probe. The determinant was identified on a 5.4-kb EcoRI fragment on R-plasmids from the 19 OT-resistant isolates of A. salmonicida. Hybridization with plasmids encoding the five classes (classes A to E) of OT resistance determinants demonstrated that the OT resistance plasmids of the 19 A. salmonicida isolates carried the class A resistance determinant. Analysis of data generated from restriction enzyme digests showed that the OT resistance plasmids were not identical; three profiles were characterized, two of which showed a high degree of homology.

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