Modulation of an AMPA-like glutamate receptor (SqGluR) gating by L- and D-aspartic acids

Euan R Brown, Stefania Piscopo, J T Chun, Maria Francone, I Mirabile, Antimo D'Aniello

Research output: Contribution to journalArticle

Abstract

L- and D-aspartic acids (L-Asp and D-Asp) are present in the majority of nervous systems. In phylogeny, significant levels have been reported in mollusc brains, particularly cephalopods. To examine the role of L- and D-Asp on a cephalopod receptor, we studied ligand gating of a squid glutamate receptor (SqGluR) expressed in HEK 239 (human embryonic kidney) cells. Under voltage clamp, application of L-glutamate (L-Glu; 1-30 mM), but not D-glutamate (D-Glu), or L- or D-Asp, evoked an inward current of 0.1 nA. L- or D-Asp (200 mu M) applied with 20 mM L-Glu, slowed the time course of activation and inactivation of the L-Glu gated current (time constant increased from 1s (L-Glu alone) to 3 s (D-Asp and L-Glu) and to 19 s (L-Asp and L-Glu)). Our results suggest that in molluscan systems, aspartic acid could act as a neuromodulator during glutamatergic transmission and could significantly alter synaptic integration by slowing glutamate receptor gating.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalAmino Acids
Volume32
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007

Cite this

Brown, Euan R ; Piscopo, Stefania ; Chun, J T ; Francone, Maria ; Mirabile, I ; D'Aniello, Antimo. / Modulation of an AMPA-like glutamate receptor (SqGluR) gating by L- and D-aspartic acids. In: Amino Acids. 2007 ; Vol. 32, No. 1. pp. 53-57.
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Brown, ER, Piscopo, S, Chun, JT, Francone, M, Mirabile, I & D'Aniello, A 2007, 'Modulation of an AMPA-like glutamate receptor (SqGluR) gating by L- and D-aspartic acids', Amino Acids, vol. 32, no. 1, pp. 53-57. https://doi.org/10.1007/s00726-006-0349-3

Modulation of an AMPA-like glutamate receptor (SqGluR) gating by L- and D-aspartic acids. / Brown, Euan R; Piscopo, Stefania; Chun, J T; Francone, Maria; Mirabile, I; D'Aniello, Antimo.

In: Amino Acids, Vol. 32, No. 1, 01.01.2007, p. 53-57.

Research output: Contribution to journalArticle

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AU - Piscopo, Stefania

AU - Chun, J T

AU - Francone, Maria

AU - Mirabile, I

AU - D'Aniello, Antimo

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AB - L- and D-aspartic acids (L-Asp and D-Asp) are present in the majority of nervous systems. In phylogeny, significant levels have been reported in mollusc brains, particularly cephalopods. To examine the role of L- and D-Asp on a cephalopod receptor, we studied ligand gating of a squid glutamate receptor (SqGluR) expressed in HEK 239 (human embryonic kidney) cells. Under voltage clamp, application of L-glutamate (L-Glu; 1-30 mM), but not D-glutamate (D-Glu), or L- or D-Asp, evoked an inward current of 0.1 nA. L- or D-Asp (200 mu M) applied with 20 mM L-Glu, slowed the time course of activation and inactivation of the L-Glu gated current (time constant increased from 1s (L-Glu alone) to 3 s (D-Asp and L-Glu) and to 19 s (L-Asp and L-Glu)). Our results suggest that in molluscan systems, aspartic acid could act as a neuromodulator during glutamatergic transmission and could significantly alter synaptic integration by slowing glutamate receptor gating.

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