TY - JOUR
T1 - Modifying glycoalkaloid content in transgenic potato - Metabolome impacts
AU - Shepherd, Louise Vida Traill
AU - Hackett, Christine Anne
AU - Alexander, Colin James
AU - McNicol, James William
AU - Sungurtas, Julia Anne
AU - Stewart, Derek
AU - McCue, Kent Frank
AU - Belknap, William Richardson
AU - Davies, Howard Vivian
PY - 2015/11/15
Y1 - 2015/11/15
N2 - Abstract Metabolite profiling has been used to assess the potential for unintended composition changes in potato (Solanum tuberosum L. cv. Desirée) tubers, which have been genetically modified (GM) to reduce glycoalkaloid content, via the independent down-regulation of three genes SGT1, SGT2 and SGT3 known to be involved in glycoalkaloid biosynthesis. Differences between the three groups of antisense lines and control lines were assessed using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS, and data analysed using principal component analysis and analysis of variance. Compared with the wild-type (WT) control, LC-MS revealed not only the expected changes in specific glycoalkaloid levels in the GM lines, but also significant changes in several other metabolites, some of which were explicable in terms of known pathways. Analysis of polar and non-polar metabolites by GC-MS revealed other significant (unintended) differences between SGT lines and the WT, but also between the WT control and other control lines used.
AB - Abstract Metabolite profiling has been used to assess the potential for unintended composition changes in potato (Solanum tuberosum L. cv. Desirée) tubers, which have been genetically modified (GM) to reduce glycoalkaloid content, via the independent down-regulation of three genes SGT1, SGT2 and SGT3 known to be involved in glycoalkaloid biosynthesis. Differences between the three groups of antisense lines and control lines were assessed using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS, and data analysed using principal component analysis and analysis of variance. Compared with the wild-type (WT) control, LC-MS revealed not only the expected changes in specific glycoalkaloid levels in the GM lines, but also significant changes in several other metabolites, some of which were explicable in terms of known pathways. Analysis of polar and non-polar metabolites by GC-MS revealed other significant (unintended) differences between SGT lines and the WT, but also between the WT control and other control lines used.
KW - Genetic modification
KW - Glycoalkaloids
KW - Metabolomics
KW - Potato (Solanum tuberosum L.)
KW - SGT
KW - Transgenic
UR - http://www.scopus.com/inward/record.url?scp=84928818141&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2015.04.111
DO - 10.1016/j.foodchem.2015.04.111
M3 - Article
C2 - 25977048
AN - SCOPUS:84928818141
SN - 0308-8146
VL - 187
SP - 437
EP - 443
JO - Food Chemistry
JF - Food Chemistry
ER -