Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Ian R Ellis; Sarah J Jones; Yvonne Lindsay; Go Ohe; Ana M Schor; Seth L Schor; Nicolas R Leslie

doi:10.1016/j.cellsig.2010.06.005

Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Ian R Ellis, Sarah J Jones, Yvonne Lindsay, Go Ohe, Ana M Schor, Seth L Schor, Nicolas R Leslie

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits AKT activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT.

Original language	English
Pages (from-to)	1655-1659
Number of pages	5
Journal	Cellular Signalling
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.cellsig.2010.06.005
Publication status	Published - Nov 2010

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title = "Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT",

abstract = "The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits AKT activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT.",

author = "Ellis, {Ian R} and Jones, {Sarah J} and Yvonne Lindsay and Go Ohe and Schor, {Ana M} and Schor, {Seth L} and Leslie, {Nicolas R}",

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AU - Ellis, Ian R

AU - Jones, Sarah J

AU - Lindsay, Yvonne

AU - Ohe, Go

AU - Schor, Ana M

AU - Schor, Seth L

AU - Leslie, Nicolas R

PY - 2010/11

Y1 - 2010/11

N2 - The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits AKT activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT.

AB - The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits AKT activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT.

U2 - 10.1016/j.cellsig.2010.06.005

DO - 10.1016/j.cellsig.2010.06.005

M3 - Article

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SN - 0898-6568

VL - 22

SP - 1655

EP - 1659

JO - Cellular Signalling

JF - Cellular Signalling

IS - 11

ER -

Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT

Abstract

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