Microfocus X-ray diffraction of historical parchment reveals variations in structural features through parchment cross sections

We propose a new method of investigating variation of preservation within a parchment sample, which allows a more detailed analysis of
alteration of the material structure. X-ray diffraction analysis of parchment typically involves the sample aligned with the plane of the parchment
perpendicular to the direction of the X-ray beam, with a beam size of approximately 200 ím and an image consisting of the composite
diffraction features from the entire thickness of the sample. Here we describe the use of microfocus X-ray beams, with a beam size of 1.5 ím
vertically ´ 15 ím horizontally, to carry out surface-to-surface scans of thin sections of parchment. Up to 200 images can be taken in a single
cross-sectional scan of a 300 ím thick parchment section. This allows for X-ray diffraction analysis of features present only in specific areas
of the parchment, such as at the surface. The orientation of collagen fibrils in the plane of the parchment, the effects of laser cleaning
(including possible laser induced damage), mineral phases and crystalline lipids present in samples, and parchment structure under an inked
region are investigated. It is shown that the long collagen fibril axis lies parallel to the parchment surface throughout the sections. Laser
cleaning appears not to damage the collagen in parchment, while laser-damaged samples display gelatinization of the collagen at the surface.
Polymorphs of calcium carbonate were detected in several samples but in most cases were not confined to the surfaces, as would be expected
if the chalk finishing process was the main source of mineral phases in parchment. Crystalline lipid is found in most samples and appears
to exhibit a preferential alignment with the plane of the phospholipid bilayer arranged parallel to the long fibril axis of collagen. The d spacing
of the lipid is variable throughout a parchment section, indicating fluctuations in the hydration state, phase, or biochemical composition of
the lipid. Ink affects the parchment to a depth of approximately 90 ím, as measured by principal components analysis, disrupting the structure
of the collagen to this depth. These features demonstrate the ability of this technique to examine diagenesis of individual components of
parchment on a scale not previously studied.