MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE

Adelaïde Lety-Stefanska; Alvaro Jose Conde; Ieva Keraite; Nicholas R. Leslie; Maïwenn Kersaudy-Kerhoas

MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE

Adelaïde Lety-Stefanska, Alvaro Jose Conde, Ieva Keraite, Nicholas R. Leslie, Maïwenn Kersaudy-Kerhoas^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Liquid biopsies allow DNA shed from tumours to be detected using blood tests without invasive and expensive surgery associated with traditional tissue biopsies. Despite these benefits, liquid biopsies remain a challenging assay to be deployed in primary oncology care as the main cancer diagnostic or screening tool, because tumour DNA is not always detectable. This poor sensitivity is largely due to the presence of a vast amount of genomic background DNA which masks the tumour DNA signal. Here we present a mixing-free approach towards the automated on-chip enrichment of circulating tumour DNA, that has the potential to reduce sequencing costs and enable point-of-care detection of mutations.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
Publisher	Chemical and Biological Microsystems Society
Pages	679-680
Number of pages	2
Volume	1
ISBN (Electronic)	9781733419031
ISBN (Print)	9781713855736
Publication status	Published - Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021 https://www.microtasconferences.org/microtas2021/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21
Internet address	https://www.microtasconferences.org/microtas2021/

Keywords

Cell-free Circulating DNA
Circulating tumour DNA
Enrichment
Sample Preparation

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

https://www.proceedings.com/64786.html

Cite this

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title = "MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE",

abstract = "Liquid biopsies allow DNA shed from tumours to be detected using blood tests without invasive and expensive surgery associated with traditional tissue biopsies. Despite these benefits, liquid biopsies remain a challenging assay to be deployed in primary oncology care as the main cancer diagnostic or screening tool, because tumour DNA is not always detectable. This poor sensitivity is largely due to the presence of a vast amount of genomic background DNA which masks the tumour DNA signal. Here we present a mixing-free approach towards the automated on-chip enrichment of circulating tumour DNA, that has the potential to reduce sequencing costs and enable point-of-care detection of mutations.",

keywords = "Cell-free Circulating DNA, Circulating tumour DNA, Enrichment, Sample Preparation",

author = "Adela{\"i}de Lety-Stefanska and Conde, {Alvaro Jose} and Ieva Keraite and Leslie, {Nicholas R.} and Ma{\"i}wenn Kersaudy-Kerhoas",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

language = "English",

isbn = "9781713855736",

volume = "1",

pages = "679--680",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)",

publisher = "Chemical and Biological Microsystems Society",

url = "https://www.microtasconferences.org/microtas2021/",

}

Lety-Stefanska, A, Conde, AJ, Keraite, I, Leslie, NR & Kersaudy-Kerhoas, M 2021, MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021). vol. 1, Chemical and Biological Microsystems Society, pp. 679-680, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, Palm Springs, Virtual, California, United States, 10/10/21. <https://www.proceedings.com/64786.html>

MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE. / Lety-Stefanska, Adelaïde; Conde, Alvaro Jose; Keraite, Ieva et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021). Vol. 1 Chemical and Biological Microsystems Society, 2021. p. 679-680.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE

AU - Lety-Stefanska, Adelaïde

AU - Conde, Alvaro Jose

AU - Keraite, Ieva

AU - Leslie, Nicholas R.

AU - Kersaudy-Kerhoas, Maïwenn

PY - 2021/10

Y1 - 2021/10

N2 - Liquid biopsies allow DNA shed from tumours to be detected using blood tests without invasive and expensive surgery associated with traditional tissue biopsies. Despite these benefits, liquid biopsies remain a challenging assay to be deployed in primary oncology care as the main cancer diagnostic or screening tool, because tumour DNA is not always detectable. This poor sensitivity is largely due to the presence of a vast amount of genomic background DNA which masks the tumour DNA signal. Here we present a mixing-free approach towards the automated on-chip enrichment of circulating tumour DNA, that has the potential to reduce sequencing costs and enable point-of-care detection of mutations.

AB - Liquid biopsies allow DNA shed from tumours to be detected using blood tests without invasive and expensive surgery associated with traditional tissue biopsies. Despite these benefits, liquid biopsies remain a challenging assay to be deployed in primary oncology care as the main cancer diagnostic or screening tool, because tumour DNA is not always detectable. This poor sensitivity is largely due to the presence of a vast amount of genomic background DNA which masks the tumour DNA signal. Here we present a mixing-free approach towards the automated on-chip enrichment of circulating tumour DNA, that has the potential to reduce sequencing costs and enable point-of-care detection of mutations.

KW - Cell-free Circulating DNA

KW - Circulating tumour DNA

KW - Enrichment

KW - Sample Preparation

UR - http://www.scopus.com/inward/record.url?scp=85136979968&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85136979968

SN - 9781713855736

VL - 1

SP - 679

EP - 680

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

MICROFLUIDIC RARE ALLELE ENRICHMENT IN CIRCULATING DNA SAMPLE

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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