Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa

John McGrath; C. Honrado; D. C. Spencer; Helen Bridle; H. Morgan

Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa

John McGrath^*, C. Honrado, D. C. Spencer, Helen Bridle, H. Morgan

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The existing method of detection for the waterborne pathogens Cryptosporidium and Giardia, EPA 1623.1, overestimates sample infectivity and gives no information with regards to the species or viability status of recovered pathogens. The ability to rapidly decipher species-level information would allow more accurate risk assessment and save numerous resources for water utilities. Microfluidic Impedance Cytometry has been reported in the literature for the detection and discrimination of various cell types at the single-cell level, making this method suitable for such environmental applications. Using low-frequency impedance, we discriminated Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia with a minimum certainty of 96%.

Original language	English
Title of host publication	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Publisher	Chemical and Biological Microsystems Society
Pages	1695-1697
Number of pages	3
Volume	1
ISBN (Print)	9781510817876
Publication status	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated title	MicroTAS 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15

Keywords

Cryptosporidium
Giardia
Microfluidic Impedance Cytometry
Waterborne Pathogens

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

@inproceedings{efd97ef668a241fda39c801da9f212df,

title = "Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa",

abstract = "The existing method of detection for the waterborne pathogens Cryptosporidium and Giardia, EPA 1623.1, overestimates sample infectivity and gives no information with regards to the species or viability status of recovered pathogens. The ability to rapidly decipher species-level information would allow more accurate risk assessment and save numerous resources for water utilities. Microfluidic Impedance Cytometry has been reported in the literature for the detection and discrimination of various cell types at the single-cell level, making this method suitable for such environmental applications. Using low-frequency impedance, we discriminated Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia with a minimum certainty of 96%.",

keywords = "Cryptosporidium, Giardia, Microfluidic Impedance Cytometry, Waterborne Pathogens",

author = "John McGrath and C. Honrado and Spencer, {D. C.} and Helen Bridle and H. Morgan",

year = "2015",

language = "English",

isbn = "9781510817876 ",

volume = "1",

pages = "1695--1697",

booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",

publisher = "Chemical and Biological Microsystems Society",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

}

McGrath, J, Honrado, C, Spencer, DC, Bridle, H & Morgan, H 2015, Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). vol. 1, Chemical and Biological Microsystems Society, pp. 1695-1697, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015, Gyeongju, Korea, Republic of, 25/10/15.

Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa. / McGrath, John; Honrado, C.; Spencer, D. C. et al.
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). Vol. 1 Chemical and Biological Microsystems Society, 2015. p. 1695-1697.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa

AU - McGrath, John

AU - Honrado, C.

AU - Spencer, D. C.

AU - Bridle, Helen

AU - Morgan, H.

PY - 2015

Y1 - 2015

N2 - The existing method of detection for the waterborne pathogens Cryptosporidium and Giardia, EPA 1623.1, overestimates sample infectivity and gives no information with regards to the species or viability status of recovered pathogens. The ability to rapidly decipher species-level information would allow more accurate risk assessment and save numerous resources for water utilities. Microfluidic Impedance Cytometry has been reported in the literature for the detection and discrimination of various cell types at the single-cell level, making this method suitable for such environmental applications. Using low-frequency impedance, we discriminated Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia with a minimum certainty of 96%.

AB - The existing method of detection for the waterborne pathogens Cryptosporidium and Giardia, EPA 1623.1, overestimates sample infectivity and gives no information with regards to the species or viability status of recovered pathogens. The ability to rapidly decipher species-level information would allow more accurate risk assessment and save numerous resources for water utilities. Microfluidic Impedance Cytometry has been reported in the literature for the detection and discrimination of various cell types at the single-cell level, making this method suitable for such environmental applications. Using low-frequency impedance, we discriminated Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia with a minimum certainty of 96%.

KW - Cryptosporidium

KW - Giardia

KW - Microfluidic Impedance Cytometry

KW - Waterborne Pathogens

UR - http://www.scopus.com/inward/record.url?scp=84983335996&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84983335996

SN - 9781510817876

VL - 1

SP - 1695

EP - 1697

BT - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

PB - Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa

Abstract

Conference

Keywords

ASJC Scopus subject areas

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