Microfluidic impedance cytometry for species-level discrimination of waterborne protozoa

John McGrath, C. Honrado, D. C. Spencer, Helen Bridle, H. Morgan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The existing method of detection for the waterborne pathogens Cryptosporidium and Giardia, EPA 1623.1, overestimates sample infectivity and gives no information with regards to the species or viability status of recovered pathogens. The ability to rapidly decipher species-level information would allow more accurate risk assessment and save numerous resources for water utilities. Microfluidic Impedance Cytometry has been reported in the literature for the detection and discrimination of various cell types at the single-cell level, making this method suitable for such environmental applications. Using low-frequency impedance, we discriminated Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia with a minimum certainty of 96%.

Original languageEnglish
Title of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
PublisherChemical and Biological Microsystems Society
Pages1695-1697
Number of pages3
Volume1
ISBN (Print)9781510817876
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2015
Abbreviated titleMicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • Cryptosporidium
  • Giardia
  • Microfluidic Impedance Cytometry
  • Waterborne Pathogens

ASJC Scopus subject areas

  • Control and Systems Engineering

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