Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples

Alvaro Jose Conde, Ieva Keraite, Nicholas R. Leslie, Maïwenn Kersaudy-Kerhoas

Research output: Chapter in Book/Report/Conference proceedingChapter


Cell-free DNA has many applications in clinical medicine, in particular in cancer diagnosis and cancer treatment monitoring. Microfluidic-based solutions could provide solutions for rapid, cheaper, decentralized detection of cell-free tumoral DNA from a simple blood draw, or liquid biopsies, replacing invasive procedures or expensive scans. In this method, we present a simple microfluidic system for the extraction of cell-free DNA from low volume of plasma samples (≤500 μL). The technique is suitable for either static or continuous flow systems and can be used as a stand-alone module or integrated within a lab-on-chip system. The system relies on a simple yet highly versatile bubble-based micromixer module whose custom components can be fabricated with a combination of low-cost rapid prototyping techniques or ordered via widely available 3D-printing services. This system is capable of performing cell-free DNA extractions from small volumes of blood plasma with up to a tenfold increase in capture efficiency when compared to control methods.

Original languageEnglish
Title of host publicationMicrofluidic Systems for Cancer Diagnosis
PublisherHumana Press
Number of pages18
ISBN (Electronic)9781071632710
ISBN (Print)9781071632703
Publication statusPublished - 11 Jun 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Micromixer
  • Microfluidics - methods
  • Liquid biopsy
  • Cell-free DNA
  • Microfluidics
  • Acoustofluidics
  • Lab-On-A-Chip Devices
  • Cell-Free Nucleic Acids
  • Oligonucleotide Array Sequence Analysis
  • Liquid Biopsy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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