@inbook{42957c54127d406d9fa78a6ad80dc1eb,
title = "Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples",
abstract = "Cell-free DNA has many applications in clinical medicine, in particular in cancer diagnosis and cancer treatment monitoring. Microfluidic-based solutions could provide solutions for rapid, cheaper, decentralized detection of cell-free tumoral DNA from a simple blood draw, or liquid biopsies, replacing invasive procedures or expensive scans. In this method, we present a simple microfluidic system for the extraction of cell-free DNA from low volume of plasma samples (≤500 μL). The technique is suitable for either static or continuous flow systems and can be used as a stand-alone module or integrated within a lab-on-chip system. The system relies on a simple yet highly versatile bubble-based micromixer module whose custom components can be fabricated with a combination of low-cost rapid prototyping techniques or ordered via widely available 3D-printing services. This system is capable of performing cell-free DNA extractions from small volumes of blood plasma with up to a tenfold increase in capture efficiency when compared to control methods.",
keywords = "Micromixer, Microfluidics - methods, Liquid biopsy, Cell-free DNA, Microfluidics, Acoustofluidics, Lab-On-A-Chip Devices, Cell-Free Nucleic Acids, Oligonucleotide Array Sequence Analysis, Liquid Biopsy",
author = "Conde, {Alvaro Jose} and Ieva Keraite and Leslie, {Nicholas R.} and Ma{\"i}wenn Kersaudy-Kerhoas",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
month = jun,
day = "11",
doi = "10.1007/978-1-0716-3271-0_11",
language = "English",
isbn = "9781071632703",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "163--180",
booktitle = "Microfluidic Systems for Cancer Diagnosis",
address = "United States",
}
