TY - JOUR
T1 - Microcystin-LR at sublethal concentrations induce rapid morphology of liver and muscle tissues in the fish species Astyanax altiparanae (Lambari)
AU - Rodrigues, Nátali Bosquê
AU - Pitol, Dimítrius Leonardo
AU - Tocchini de Figueiredo, Fellipe Augusto
AU - Tenfen Das Chagas Lima, Ana Claudia
AU - Henry, Theodore B.
AU - Mardegan Issa, João Paulo
AU - de Aragão Umbuzeiro, Gisela
AU - Pereira, Bruno Fiorelini
N1 - Funding Information:
The present work was financially supported by the São Paulo Research Foundation (FAPESP) process number: 2019/24438–1 and 2016/02946–7 .
Funding Information:
The authors are thankful to FAPESP process number: 2016/02946–7 and 2019/24438–1 for financial support and to ICMBio / CEPTA – Institute Chico Mendes for providing the specimens used in this experiment. The authors are thankful to the reviewers and editors of Toxicon for all their contribution in correcting and improving the text.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/5
Y1 - 2022/5
N2 - The process of eutrophication and consequent proliferation of cyanobacteria in rivers and lakes leads to increasing numbers of harmful algal blooms and higher concentration of toxic metabolites in freshwater bodies. Microcystin is a toxic metabolite produced by cyanobacteria that is frequently detected and can pose health risks to important freshwater species including fish. The aim of the present study was to evaluate the effects of microcystin-LR on the morphology of Astyanax altiparanae's liver and muscle. One hundred (n = 100) Astyanax altiparanae were divided into 5 groups (n = 20) with 24 h and 96 h of microcystin exposition at two doses of 0.5 and 1.0 μg/L. Differences were observed in the microcystin treatment with respect to histopathological analyses including cytoplastic degradation, displacement, and increase in nuclei volume and area of hepatocytes. Hyperemia and dilation of blood capillaries were seen in the liver. There were also observable changes in the size of muscle fibers and muscle inflammation. Our results demonstrate that microcystins can impact the integrity of both tissues even at sublethal concentrations. Low doses of microcystins are therefore sufficient to intoxicate fish livers and muscle tissues.
AB - The process of eutrophication and consequent proliferation of cyanobacteria in rivers and lakes leads to increasing numbers of harmful algal blooms and higher concentration of toxic metabolites in freshwater bodies. Microcystin is a toxic metabolite produced by cyanobacteria that is frequently detected and can pose health risks to important freshwater species including fish. The aim of the present study was to evaluate the effects of microcystin-LR on the morphology of Astyanax altiparanae's liver and muscle. One hundred (n = 100) Astyanax altiparanae were divided into 5 groups (n = 20) with 24 h and 96 h of microcystin exposition at two doses of 0.5 and 1.0 μg/L. Differences were observed in the microcystin treatment with respect to histopathological analyses including cytoplastic degradation, displacement, and increase in nuclei volume and area of hepatocytes. Hyperemia and dilation of blood capillaries were seen in the liver. There were also observable changes in the size of muscle fibers and muscle inflammation. Our results demonstrate that microcystins can impact the integrity of both tissues even at sublethal concentrations. Low doses of microcystins are therefore sufficient to intoxicate fish livers and muscle tissues.
KW - Eutrophication
KW - Fish
KW - Harmful algal blooms
KW - Microcystin
KW - Water pollution
UR - http://www.scopus.com/inward/record.url?scp=85127044005&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2022.03.006
DO - 10.1016/j.toxicon.2022.03.006
M3 - Article
C2 - 35306038
SN - 0041-0101
VL - 211
SP - 70
EP - 78
JO - Toxicon
JF - Toxicon
ER -
