Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)

D. H. Kim, J. Brunt, B. Austin

    Research output: Contribution to journalArticle

    Abstract

    Aims: The aim of this study was to understand the microbial community of intestinal contents and mucosal layer in the intestine of rainbow trout by means of culture-dependent conventional and independent molecular techniques. Methods and Results: Forty-one culturable microbial phylotypes, and 39 sequences from 16S rRNA and two from 18S rRNA genes, were retrieved. Aeromonadaceae, Enterobacteriaceae and Pseudomonadaceae representatives were the dominant cultured bacteria. Genomic DNA isolated from intestinal contents and mucus was used to generate 104 random clones, which were grouped into 32 phylotypes at 99% minimum similarity, most of which were affiliated with Proteobacteria (>70% of the total). However, unlike library C (intestinal contents), the phyla Bacteroidetes and Fusobacteria were not found in intestinal mucus (library M), indicating that the microbiota in the gut mucus was different from that of the intestinal contents. Twelve sequences were retrieved from denaturing gradient gel electrophoresis analysis, and dominant bands were mostly related to Clostridium. Conclusions: Many novel sequences that have not been previously recognized as part of the intestinal flora of rainbow trout were retrieved. Significance and Impact of the Study: The fish gut harbours a larger bacterial diversity than previously recognized, and the diversity of gut mucus is different from that of intestinal contents. © 2007 The Authors.

    Original languageEnglish
    Pages (from-to)1654-1664
    Number of pages11
    JournalJournal of Applied Microbiology
    Volume102
    Issue number6
    DOIs
    Publication statusPublished - Jun 2007

    Fingerprint

    Gastrointestinal Contents
    Oncorhynchus mykiss
    Mucus
    Aeromonadaceae
    Libraries
    Pseudomonadaceae
    Fusobacteria
    Bacteroidetes
    Proteobacteria
    Denaturing Gradient Gel Electrophoresis
    Clostridium
    Enterobacteriaceae
    rRNA Genes
    Intestines
    Fishes
    Clone Cells
    Bacteria
    DNA

    Keywords

    • 16S rDNA
    • Biodiversity
    • Intestinal microflora
    • Intestinal mucus
    • Rainbow trout

    Cite this

    Kim, D. H. ; Brunt, J. ; Austin, B. / Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss). In: Journal of Applied Microbiology. 2007 ; Vol. 102, No. 6. pp. 1654-1664.
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    Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss). / Kim, D. H.; Brunt, J.; Austin, B.

    In: Journal of Applied Microbiology, Vol. 102, No. 6, 06.2007, p. 1654-1664.

    Research output: Contribution to journalArticle

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