Microbial degradation of Benzyldimethyl Hexadecylammonium Chloride (BDHAC) used in oilfield chemical formulations

D. E. Bassey, S. J W Grigson, W. J. Mitchell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Following enrichment in its presence, four strains of bacteria, isolated from marine sediments, were shown to degrade the quaternary ammonium surfactant Benzyldimethyl Hexadecylammonium Chloride (BDHAC) in a minimal salts medium. The bacteria were identified by 16S rDNA sequencing, were shown to belong to several genera and were identified as Bacillus niabensis, Bacillus subtilis, Thalassospira sp, and Sporosarcina sp. Initial investigations have shown that the bacteria are capable of degrading BDHAC when it is present at concentrations in the range 2-4 mg/ml. In media containing BDHAC, up to 90% was degraded within 7 days, but this supported only limited growth of the bacteria. Preliminary analysis of samples after degradation experiment by ESI-MS/MS produced a peak with a parent-daughter ion transition of 136.0-91.0, corresponding to N, N-dimethylbenzylamine. The presence of this potential metabolite suggests the cleavage of the C alkyl-N bond as a step in BDHAC catabolism. Copyright 2009, Society of Petroleum Engineers.

Original languageEnglish
Title of host publicationSPE Americas E and P Environmental and Safety Conference 2009
Pages452-462
Number of pages11
Publication statusPublished - 2009
EventSPE Americas E and P Environmental and Safety Conference 2009 - San Antonio, TX, United States
Duration: 23 Mar 200925 Mar 2009

Conference

ConferenceSPE Americas E and P Environmental and Safety Conference 2009
CountryUnited States
CitySan Antonio, TX
Period23/03/0925/03/09

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    Bassey, D. E., Grigson, S. J. W., & Mitchell, W. J. (2009). Microbial degradation of Benzyldimethyl Hexadecylammonium Chloride (BDHAC) used in oilfield chemical formulations. In SPE Americas E and P Environmental and Safety Conference 2009 (pp. 452-462)