Method and device

Research output: Patent

Abstract

The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13' at the 3'-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23 at the 3'-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25 at the 5'-end of the nucleic acid sequence, wherein the 5'-end linker sequence L25 of nucleic acid sequence N2 is complementary to the 3'-end linker sequence L13 of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23at the 3'-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3'-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3'-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5'-end, wherein for the first additional nucleic acid sequence N3 the 5'-end linker sequence L35 is complementary to the 3'-end linker sequence L23' of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5'-end linker sequence is complementary to the 3'-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence; wherein at least step (iv) is carried out on a microfluidic device.
Original languageEnglish
Patent number1222
IPCC12Q 1/68 (2006.01)
Publication statusPublished - 29 Dec 2011

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Nucleic acid sequences
Oligonucleotides
Acids

Cite this

Kersaudy-Kerhoas, M. (2011). IPC No. C12Q 1/68 (2006.01). Method and device. (Patent No. 1222).
Kersaudy-Kerhoas, Maïwenn (Inventor). / Method and device. IPC No.: C12Q 1/68 (2006.01). Patent No.: 1222.
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Kersaudy-Kerhoas, M 2011, Method and device, Patent No. 1222, IPC No. C12Q 1/68 (2006.01).

Method and device. / Kersaudy-Kerhoas, Maïwenn (Inventor).

IPC No.: C12Q 1/68 (2006.01). Patent No.: 1222.

Research output: Patent

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T1 - Method and device

AU - Kersaudy-Kerhoas, Maïwenn

PY - 2011/12/29

Y1 - 2011/12/29

N2 - The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13' at the 3'-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23 at the 3'-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25 at the 5'-end of the nucleic acid sequence, wherein the 5'-end linker sequence L25 of nucleic acid sequence N2 is complementary to the 3'-end linker sequence L13 of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23at the 3'-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3'-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3'-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5'-end, wherein for the first additional nucleic acid sequence N3 the 5'-end linker sequence L35 is complementary to the 3'-end linker sequence L23' of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5'-end linker sequence is complementary to the 3'-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence; wherein at least step (iv) is carried out on a microfluidic device.

AB - The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13' at the 3'-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23 at the 3'-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25 at the 5'-end of the nucleic acid sequence, wherein the 5'-end linker sequence L25 of nucleic acid sequence N2 is complementary to the 3'-end linker sequence L13 of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23at the 3'-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3'-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3'-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5'-end, wherein for the first additional nucleic acid sequence N3 the 5'-end linker sequence L35 is complementary to the 3'-end linker sequence L23' of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5'-end linker sequence is complementary to the 3'-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence; wherein at least step (iv) is carried out on a microfluidic device.

M3 - Patent

M1 - 1222

ER -

Kersaudy-Kerhoas M, inventor. Method and device. C12Q 1/68 (2006.01). 2011 Dec 29.