Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sørfjord, Norway)

Ana I Catarino; Henrique N. Cabral; Kris Peeters; Philippe Pernet; Usha Punjabi; Philippe Dubois

doi:10.1897/07-402.1

Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sørfjord, Norway)

Ana I Catarino, Henrique N. Cabral, Kris Peeters, Philippe Pernet, Usha Punjabi, Philippe Dubois

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.

Original language	English
Pages (from-to)	1553-1560
Number of pages	8
Journal	Environmental Toxicology and Chemistry
Volume	27
Issue number	7
DOIs	https://doi.org/10.1897/07-402.1
Publication status	Published - Jul 2008

Keywords

Asterias rubens
Echinus acutus
Metals
RNA/DNA ratio
Sperm motility

ASJC Scopus subject areas

General Environmental Science
Environmental Chemistry
Toxicology
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1897/07-402.1

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title = "Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the S{\o}rfjord, Norway)",

abstract = "The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the S{\o}rfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The S{\o}rfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.",

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author = "Catarino, {Ana I} and Cabral, {Henrique N.} and Kris Peeters and Philippe Pernet and Usha Punjabi and Philippe Dubois",

year = "2008",

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AU - Catarino, Ana I

AU - Cabral, Henrique N.

AU - Peeters, Kris

AU - Pernet, Philippe

AU - Punjabi, Usha

AU - Dubois, Philippe

PY - 2008/7

Y1 - 2008/7

N2 - The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.

AB - The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.

KW - Asterias rubens

KW - Echinus acutus

KW - Metals

KW - RNA/DNA ratio

KW - Sperm motility

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U2 - 10.1897/07-402.1

DO - 10.1897/07-402.1

M3 - Article

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SN - 0730-7268

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EP - 1560

JO - Environmental Toxicology and Chemistry

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ER -

Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sørfjord, Norway)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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