Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sørfjord, Norway)

Ana I Catarino, Henrique N. Cabral, Kris Peeters, Philippe Pernet, Usha Punjabi, Philippe Dubois

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.

    Original languageEnglish
    Pages (from-to)1553-1560
    Number of pages8
    JournalEnvironmental Toxicology and Chemistry
    Volume27
    Issue number7
    DOIs
    Publication statusPublished - Jul 2008

    Keywords

    • Asterias rubens
    • Echinus acutus
    • Metals
    • RNA/DNA ratio
    • Sperm motility

    ASJC Scopus subject areas

    • Environmental Science(all)
    • Environmental Chemistry
    • Toxicology
    • Health, Toxicology and Mutagenesis

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