Metal binding properties of the EPS produced by Halomonas sp. TG39 and its potential in enhancing trace element bioavailability to eukaryotic phytoplankton

Tony Gutierrez, Dondra Biller, Tracy Shimmield, David Green

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    An emergent property of exopolysaccharides (EPS) produced by marine bacteria is their net negative charge, predominantly conferred by their high uronic acids content. Here, we investigated the EPS produced by an algal-associated marine bacterium, Halomonas sp. strain TG39, for its capacity to sequester trace metals and mediate their bioavailability to eukaryotic phytoplankton. Metal analysis of the purified EPS revealed that it contained high levels of K, Ca,Mg and several essential trace metals, including Zn, Cu, Fe and the metalloid Si. Desorption experiments with marine sediment showed that the EPS possessed a specific binding capacity for Ca, Si, Fe, Mn, Mg and Al. Depending on the ionic conditions, Fe was the third or fourth most highly-adsorbed metal out of 27 elements analyzed. Experiments employing Fe-limited synthetic ocean seawater showed that growth of the marine diatom Thalassiosira weissflogii (axenic
    strain) was enhanced when incubated in the presence of either purified EPS or EPS that had been preexposed to marine sediment, compared to non-EPS
    amended controls. This growth enhancement was attributed to the EPS binding and increasing the bioavailability of key trace metal elements, such as Fe(III). Since the bacterium used in this study was originally isolated from a marine micro-alga, this work highlights the possibility that bacterial associates of eukaryotic algae could be influencing the bioavailability of Fe(III) to phytoplankton via their production of polyanionic EPS. More widely, this work reinforces the potential importance of marine bacterial EPS in trace metal biogeochemical cycling.
    Original languageEnglish
    Pages (from-to)1185-1194
    Number of pages10
    JournalBioMetals
    Volume25
    Issue number6
    DOIs
    Publication statusPublished - Dec 2012

    Keywords

    • Exopolysaccharide
    • iron
    • Metal ions
    • eukaryotic phytoplankton
    • Marine bacteria

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