Metabolism in marine flatfish-II. Protein digestion in dover sole (Solea solea L.)

J. Clark, N. L. MacDonald, J. R. Stark

    Research output: Contribution to journalArticle

    Abstract

    1. 1. The digestive tracts of adult and juvenile Dover sole were examined for protease activities. 2. 2. A pepsin-like protease with an optimal pH value of 1.7 predominated in the stomach region, but the main endoprotease action in the foregut, midgut and hindgut regions was optimal in the range of pH 9.5-10.5 and showed good activity towards elastin orcein. 3. 3. Experiments using synthetic substrates suggested the presence of chymotrypsin- and trypsin-like activities optimal between pH 7 and 8. Collagenase activity was also shown to exist in this pH region. 4. 4. The presence of enzymes corresponding to carboxypeptidases a and b and leucine aminopeptidase was indicated. 5. 5. The possible significance of these results to the farming of Dover sole is discussed. © 1985.

    Original languageEnglish
    Pages (from-to)217-222
    Number of pages6
    JournalComparative Biochemistry and Physiology Part B: Comparative Biochemistry
    Volume81
    Issue number1
    Publication statusPublished - 1985

    Fingerprint

    Solea solea
    Pleuronectiformes
    digestion
    metabolism
    proteinases
    proteins
    carboxypeptidases
    elastin
    leucyl aminopeptidase
    collagenase
    foregut
    hindgut
    chymotrypsin
    pepsin
    midgut
    digestive tract
    trypsin
    stomach
    farming systems
    enzymes

    Cite this

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    abstract = "1. 1. The digestive tracts of adult and juvenile Dover sole were examined for protease activities. 2. 2. A pepsin-like protease with an optimal pH value of 1.7 predominated in the stomach region, but the main endoprotease action in the foregut, midgut and hindgut regions was optimal in the range of pH 9.5-10.5 and showed good activity towards elastin orcein. 3. 3. Experiments using synthetic substrates suggested the presence of chymotrypsin- and trypsin-like activities optimal between pH 7 and 8. Collagenase activity was also shown to exist in this pH region. 4. 4. The presence of enzymes corresponding to carboxypeptidases a and b and leucine aminopeptidase was indicated. 5. 5. The possible significance of these results to the farming of Dover sole is discussed. {\circledC} 1985.",
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    Metabolism in marine flatfish-II. Protein digestion in dover sole (Solea solea L.). / Clark, J.; MacDonald, N. L.; Stark, J. R.

    In: Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol. 81, No. 1, 1985, p. 217-222.

    Research output: Contribution to journalArticle

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    AB - 1. 1. The digestive tracts of adult and juvenile Dover sole were examined for protease activities. 2. 2. A pepsin-like protease with an optimal pH value of 1.7 predominated in the stomach region, but the main endoprotease action in the foregut, midgut and hindgut regions was optimal in the range of pH 9.5-10.5 and showed good activity towards elastin orcein. 3. 3. Experiments using synthetic substrates suggested the presence of chymotrypsin- and trypsin-like activities optimal between pH 7 and 8. Collagenase activity was also shown to exist in this pH region. 4. 4. The presence of enzymes corresponding to carboxypeptidases a and b and leucine aminopeptidase was indicated. 5. 5. The possible significance of these results to the farming of Dover sole is discussed. © 1985.

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