TY - JOUR
T1 - Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut
AU - Siddiqui, Ruqaiyyah
AU - Akbar, Noor
AU - Soares, Nelson Cruz
AU - Al-Hroub, Hamza Mohammad
AU - Semreen, Mohammad Harb
AU - Maciver, Sutherland K.
AU - Khan, Naveed Ahmed
N1 - © 2023 The Authors.
PY - 2023/6
Y1 - 2023/6
N2 - AIM: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties.MATERIALS & METHODS: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry.RESULTS & CONCLUSION: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC-MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma-terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.
AB - AIM: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties.MATERIALS & METHODS: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry.RESULTS & CONCLUSION: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC-MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma-terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.
U2 - 10.2144/fsoa-2023-0030
DO - 10.2144/fsoa-2023-0030
M3 - Article
C2 - 37180607
SN - 2056-5623
VL - 9
JO - Future Science OA
JF - Future Science OA
IS - 5
M1 - FSO861
ER -
