Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut

Ruqaiyyah Siddiqui, Noor Akbar, Nelson Cruz Soares, Hamza Mohammad Al-Hroub, Mohammad Harb Semreen, Sutherland K. Maciver, Naveed Ahmed Khan

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

AIM: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties.

MATERIALS & METHODS: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry.

RESULTS & CONCLUSION: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC-MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma-terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.

Original languageEnglish
Article numberFSO861
JournalFuture Science OA
Volume9
Issue number5
Early online date2 May 2023
DOIs
Publication statusPublished - Jun 2023

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