Abstract
The trifunctional FAS2 gene encoding subunits α of the Saccharomyces cerevisiae fatty acid synthetase complex was mapped on the left arm of chromosome XVI 24 centinorgans proximal to GAL4 and 39 centimorgans distal and PEP4 relative to the centromere. Mapping was achieved by three‐independent methods: meiotic co‐segragation of FAS2 and ARO7 in recombination‐deficient spo11‐mutants; tetrad analysis of crosses between FAS2, GAL4 and PEP4; and Southern hybridization of purified FAS2 DBA with individual yeast chromosomes separated by pulsed‐field gel electrophoresis.
| Original language | English |
|---|---|
| Pages (from-to) | 411-415 |
| Number of pages | 5 |
| Journal | Yeast |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1990 |
Keywords
- Chromosome hybridisation
- Multifunctional FAS2 gene
- spo11 mapping
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Genetics
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