Lipid-linked saccharides formed during pullulan biosynthesis in Aureobasidium pullulans

Brian J. Catley, William McDowel

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    38 Citations (Scopus)


    Radioactive glycolipids were extracted from cells of Aureobasidium pullulanspulsed with d-[14C]glucose. Labelled, alkali-stable lipids were resolved into one neutral and two acidic fractions. The neutral fraction was stable to mild hydrolysis with acid, whereas the acidic fractions could be hydrolysed, yielding d-glucose and a series of oligosaccharides having mobilities corresponding to those of isomaltose, panose, and isopanose. Amyloglucosidase (EC catalysed the hydrolysis of 60% of the liberated radioactive oligosaccharides to d-glucose, indicating the presence of (1?4)-a- and (1 ? 6)-a-d-glucosidic bonds. Since these lipid-linked saccharides are produced during pullulan biosynthesis in A. pullulans, it is proposed that they are intermediates in the biosynthetic pathway of that extracellular polysaccharide. A mechanism incorporating these glycolipids into a possible scheme of polysaccharide assembly is presented. © 1982 Elsevier Scientific Publishing Company.

    Original languageEnglish
    Pages (from-to)65-75
    Number of pages11
    JournalCarbohydrate Research
    Issue number1
    Publication statusPublished - 1 May 1982


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