Limitation of spiral microchannels for particle separation in heterogeneous mixtures: Impact of particles' size and deformability

Ewa Guzniczak, Timm Krüger, Helen Bridle, Melanie Jimenez

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

Spiral microchannels have shown promising results for separation applications. Hydrodynamic particle-particle interactions are a known factor strongly influencing focusing behaviors in inertial devices, with recent work highlighting how the performance of bidisperse mixtures is altered when compared with pure components in square channels. This phenomenon has not been previously investigated in detail for spiral channels. Here, we demonstrate that, in spiral channels, both the proportion and deformability of larger particles (13 μm diameter) impact upon the recovery (up to 47% decrease) of small rigid particles (4 μm). The effect, observed at low concentrations (volume fraction <0.0012), is attributed to the hydrodynamic capture of beads by larger cells. These changes in particles focusing behavior directly impede the efficiency of the separation-diverting beads from locations expected from measurements with pure populations to co-collection with larger cells-and could hamper deployment of technology for certain applications. Similar focusing behavior alterations were noted when working with purification of stem cell end products.

Original languageEnglish
Article number044113
JournalBiomicrofluidics
Volume14
Issue number4
DOIs
Publication statusPublished - Jul 2020

ASJC Scopus subject areas

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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