Label-free and real-time monitoring of yeast cell growth by the bending of polymer microcantilever biosensors

Yifan Liu, Lilian Mary Schweizer, Wenxing Wang, Robert Lewis Reuben, Michael Schweizer, Wenmiao Shu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We demonstrate a new strategy for the label-free monitoring of yeast cell growth and its interaction with drugs using simple bending-mode of polymer microcantilever biosensors. Saccharomyces cerevisiae strains YN94-1 and YN94-19 were deposited onto microcantilever sensor surfaces and the growth curves corresponding to the bending were investigated under different culture conditions. The real-time polymer microcantilever bending signal revealed distinct growth characteristics of cells cultured in Synthetic Complete (SC) medium with and without the addition of uracil and 5'-fluororotic acid, which are indiscernible using conventional screening methods. In addition, cantilever deflection signals of up to similar to 7 mu m were observed, which can only be explained through surface stress interactions between yeast cells and the polymer cantilevers due to the enhanced sensitivity of the viscoelastic polymer cantilevers. This new method of enhanced sensitivity in static mode microcantilever monitoring of cell growth opens opportunities for highly sensitive applications in the fields of disease diagnosis and drug discovery and testing. (c) 2013 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalSensors and Actuators B: Chemical
Volume178
Early online date16 Jan 2013
DOIs
Publication statusPublished - 1 Mar 2013

Keywords

  • Microcantilever biosensors
  • Yeast growth
  • Surface stress
  • Static bending mode
  • Drug screening
  • MICROMECHANICAL CANTILEVER ARRAY
  • DNA HYBRIDIZATION
  • SINGLE CELLS
  • MASS
  • SENSORS
  • PROTEIN
  • ATTACHMENT
  • ANTIBODY
  • TENSION
  • DRIVEN

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