Kinetics of protein expression in single cells using microfluidics

Jung U. Shim, Luis Olguin, Graeme Whyte, Dan Bratton, Florian Hollfelder, Chris Abell, Wilhelm Huck

Research output: Contribution to conferencePaper

Abstract

We have coexpressed two target proteins, alkaline phosphatase(AP)[1] and Red Fluorescent Protein(mRFP1)[3], in cells while they have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for droplet storage[2]. We interrogate the enzymatic activity of AP and the expression of mRFP1 by following the fluorescence of stored droplets.

Original languageEnglish
Pages1940-1942
Number of pages3
Publication statusPublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008 - San Diego, United States
Duration: 12 Oct 200816 Oct 2008

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008
Abbreviated titleMicroTAS 2008
CountryUnited States
CitySan Diego
Period12/10/0816/10/08

Keywords

  • Droplet
  • Kinetics
  • Microfluidics
  • Protein expression

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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