Kinetics of protein expression in single cells using microfluidics

Jung U. Shim; Luis Olguin; Graeme Whyte; Dan Bratton; Florian Hollfelder; Chris Abell; Wilhelm Huck

Kinetics of protein expression in single cells using microfluidics

Jung U. Shim, Luis Olguin, Graeme Whyte, Dan Bratton, Florian Hollfelder, Chris Abell, Wilhelm Huck

Research output: Contribution to conference › Paper › peer-review

Abstract

We have coexpressed two target proteins, alkaline phosphatase(AP)[1] and Red Fluorescent Protein(mRFP1)[3], in cells while they have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for droplet storage[2]. We interrogate the enzymatic activity of AP and the expression of mRFP1 by following the fluorescence of stored droplets.

Original language	English
Pages	1940-1942
Number of pages	3
Publication status	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008 - San Diego, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008
Abbreviated title	MicroTAS 2008
Country/Territory	United States
City	San Diego
Period	12/10/08 → 16/10/08

Keywords

Droplet
Kinetics
Microfluidics
Protein expression

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Kinetics of protein expression in single cells using microfluidics",

abstract = "We have coexpressed two target proteins, alkaline phosphatase(AP)[1] and Red Fluorescent Protein(mRFP1)[3], in cells while they have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for droplet storage[2]. We interrogate the enzymatic activity of AP and the expression of mRFP1 by following the fluorescence of stored droplets.",

keywords = "Droplet, Kinetics, Microfluidics, Protein expression",

author = "Shim, {Jung U.} and Luis Olguin and Graeme Whyte and Dan Bratton and Florian Hollfelder and Chris Abell and Wilhelm Huck",

year = "2008",

language = "English",

pages = "1940--1942",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Kinetics of protein expression in single cells using microfluidics

AU - Shim, Jung U.

AU - Olguin, Luis

AU - Whyte, Graeme

AU - Bratton, Dan

AU - Hollfelder, Florian

AU - Abell, Chris

AU - Huck, Wilhelm

PY - 2008

Y1 - 2008

N2 - We have coexpressed two target proteins, alkaline phosphatase(AP)[1] and Red Fluorescent Protein(mRFP1)[3], in cells while they have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for droplet storage[2]. We interrogate the enzymatic activity of AP and the expression of mRFP1 by following the fluorescence of stored droplets.

AB - We have coexpressed two target proteins, alkaline phosphatase(AP)[1] and Red Fluorescent Protein(mRFP1)[3], in cells while they have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for droplet storage[2]. We interrogate the enzymatic activity of AP and the expression of mRFP1 by following the fluorescence of stored droplets.

KW - Droplet

KW - Kinetics

KW - Microfluidics

KW - Protein expression

UR - http://www.scopus.com/inward/record.url?scp=84902504212&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902504212

SP - 1940

EP - 1942

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Kinetics of protein expression in single cells using microfluidics

Abstract

Conference

Keywords

ASJC Scopus subject areas

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Cite this